User contributions for Iharvey

10 June 2016

• 21:4121:41, 10 June 2016 diff hist +1,211 Chromatin Immunoprecipitation for Tissue Samples No edit summary
• 20:5920:59, 10 June 2016 diff hist +5,555 N Chromatin Immunoprecipitation for Tissue Samples Created page with "The tissue fixation portion of this protocol was taken from the Ren Lab ENCODE protocol for brown adipose tissue and can be found here [https://www.encodeproject.org/document..."
• 20:4720:47, 10 June 2016 diff hist +36 Chromatin Immunoprecipitation No edit summary

17 May 2016

• 17:1117:11, 17 May 2016 diff hist −1 Designing Clones Using Benchling No edit summary current
• 17:1017:10, 17 May 2016 diff hist +2,380 N Designing Clones Using Benchling Created page with "First, go to [https://benchling.com] to create an account. The following protocol is a tutorial for designing clones using the Gibson method: #Click the "Create+" tab at the t..."

23 March 2016

• 21:3421:34, 23 March 2016 diff hist +1 Chromatin Immunoprecipitation →Incubate bead-antibody complex with fragmented, cross-linked chromatin
• 21:3321:33, 23 March 2016 diff hist +46 Chromatin Immunoprecipitation →Incubate bead-antibody complex with fragmented, cross-linked chromatin
• 19:5819:58, 23 March 2016 diff hist +16 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 19:5719:57, 23 March 2016 diff hist +255 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 19:5019:50, 23 March 2016 diff hist +24 Chromatin Immunoprecipitation →Purification of ChIP DNA

21 March 2016

• 20:4620:46, 21 March 2016 diff hist −20 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 20:4520:45, 21 March 2016 diff hist −11 Chromatin Immunoprecipitation →Incubate bead-antibody complex with fragmented, cross-linked chromatin
• 20:4320:43, 21 March 2016 diff hist −15 Chromatin Immunoprecipitation →Reverse cross-linking and recover ChIP DNA
• 20:4220:42, 21 March 2016 diff hist +3 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 20:3820:38, 21 March 2016 diff hist +32 Chromatin Immunoprecipitation →Buffers and Solutions Needed
• 20:3720:37, 21 March 2016 diff hist +6 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 20:3720:37, 21 March 2016 diff hist −4 Chromatin Immunoprecipitation →Incubate bead-antibody complex with fragmented, cross-linked chromatin
• 20:3720:37, 21 March 2016 diff hist +6 Chromatin Immunoprecipitation →Reverse cross-linking and recover ChIP DNA
• 20:3620:36, 21 March 2016 diff hist −25 Chromatin Immunoprecipitation →Analysis of Immunoprecipitated DNA
• 20:3620:36, 21 March 2016 diff hist +1,117 Chromatin Immunoprecipitation →Reverse cross-linking and recover ChIP DNA
• 20:2520:25, 21 March 2016 diff hist +184 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 20:1920:19, 21 March 2016 diff hist +76 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 20:1520:15, 21 March 2016 diff hist +22 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 20:1220:12, 21 March 2016 diff hist +22 Chromatin Immunoprecipitation →Buffers and Solutions Needed
• 20:0920:09, 21 March 2016 diff hist +12 Chromatin Immunoprecipitation →Incubate bead-antibody complex with fragmented, cross-linked chromatin
• 20:0020:00, 21 March 2016 diff hist −1 Chromatin Immunoprecipitation →Buffers and Solutions Needed
• 19:5919:59, 21 March 2016 diff hist +27 Chromatin Immunoprecipitation →Buffers and Solutions Needed

1 February 2016

• 22:4022:40, 1 February 2016 diff hist +51 Assessing isoproterenol-stimulated whole-body lipolysis in vivo →Experimental protocol

28 January 2016

• 18:1318:13, 28 January 2016 diff hist −1 Preparation of RNA Samples from Mouse Tissues →Protocol
• 18:1318:13, 28 January 2016 diff hist +40 Preparation of RNA Samples from Mouse Tissues →Protocol

26 January 2016

• 20:2820:28, 26 January 2016 diff hist 0 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 18:1718:17, 26 January 2016 diff hist +5 Chromatin Immunoprecipitation →Incubate bead-antibody complex with fragmented, cross-linked chromatin
• 18:1518:15, 26 January 2016 diff hist +294 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 18:0118:01, 26 January 2016 diff hist −1 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 18:0018:00, 26 January 2016 diff hist +73 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA

25 January 2016

• 17:1917:19, 25 January 2016 diff hist −23 RT-PCR primer design for ChIP No edit summary
• 17:1817:18, 25 January 2016 diff hist 0 RT-PCR primer design for ChIP No edit summary
• 17:1717:17, 25 January 2016 diff hist +6 RT-PCR primer design for ChIP No edit summary
• 17:1517:15, 25 January 2016 diff hist +1,524 N Locating ChIP-seq peaks from ENCODE Created page with "# Go to the UCSC website [https://genome.ucsc.edu/index.html] # Go to the ENCODE option on the left-hand side of the page # Click on the Explore ENCODE data at UCSC picture #..." current
• 16:4916:49, 25 January 2016 diff hist +38 Chromatin Immunoprecipitation →Analysis of Immunoprecipitated DNA
• 16:4416:44, 25 January 2016 diff hist +1,321 N RT-PCR primer design for ChIP Created page with "# Locate the potential gene regions you believe your protein is bound to (for glucocorticoid-induced ChIP-seq peaks refer to Locating ChIP-seq Peaks protocol). Make sure this..."

20 January 2016

• 18:5618:56, 20 January 2016 diff hist 0 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 18:5518:55, 20 January 2016 diff hist −1 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 18:0918:09, 20 January 2016 diff hist −12 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 18:0618:06, 20 January 2016 diff hist +15 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 16:5716:57, 20 January 2016 diff hist +1 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 16:5616:56, 20 January 2016 diff hist +4 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 16:5316:53, 20 January 2016 diff hist +69 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 16:3616:36, 20 January 2016 diff hist +581 Chromatin Immunoprecipitation →Immunoprecipitation
• 16:2716:27, 20 January 2016 diff hist +1,683 Chromatin Immunoprecipitation →Immunoprecipitation