User contributions for Iharvey

10 May 2018

• 15:2315:23, 10 May 2018 diff hist +48 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads current
• 15:2115:21, 10 May 2018 diff hist +6 Chromatin Immunoprecipitation →Prior to starting this section:
• 15:2015:20, 10 May 2018 diff hist +18 Chromatin Immunoprecipitation →Reverse Crosslinks of Protein/DNA Complexes to Free DNA

10 January 2018

• 17:3417:34, 10 January 2018 diff hist +2 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 17:3317:33, 10 January 2018 diff hist +122 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads

9 January 2018

• 22:1322:13, 9 January 2018 diff hist −8 Chromatin Immunoprecipitation →Prior to starting this section:
• 22:0922:09, 9 January 2018 diff hist 0 ChIP Elution Buffer No edit summary current
• 22:0922:09, 9 January 2018 diff hist +313 ChIP Elution Buffer No edit summary
• 21:4121:41, 9 January 2018 diff hist +6 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 21:4121:41, 9 January 2018 diff hist +2 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 21:3221:32, 9 January 2018 diff hist +57 Chromatin Immunoprecipitation →Before You Start
• 21:2921:29, 9 January 2018 diff hist +337 Dilution Buffer No edit summary current
• 20:1720:17, 9 January 2018 diff hist +71 N ChIP Elution Buffer Created page with "10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water."
• 20:1520:15, 9 January 2018 diff hist +79 N Dilution Buffer Created page with "0.01% SDS, 1.1% Triton X- 100, 1.2mM EDTA, 16.7mM Tris-HCl, pH 8.1, 167mM NaCl."
• 20:1520:15, 9 January 2018 diff hist +22 Chromatin Immunoprecipitation →Buffers and Solutions Needed
• 20:1220:12, 9 January 2018 diff hist +139 Chromatin Immunoprecipitation →Buffers and Solutions Needed
• 20:1120:11, 9 January 2018 diff hist −79 Chromatin Immunoprecipitation →Buffers and Solutions Needed
• 20:0920:09, 9 January 2018 diff hist +196 N TE Buffer Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location |- | Tris|| 10mM|| 150uL || 1M || Solutions Shelf |- | EDTA || 1mM || 30uL || 500mM..." current
• 20:0720:07, 9 January 2018 diff hist +4 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 20:0520:05, 9 January 2018 diff hist +402 N High Salt Immune Complex Wash Buffer Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location |- | SDS || 0.1% || 150uL || 10% || Solutions Shelf |- | Triton X-100 || 1% || 1.5mL..." current
• 19:5919:59, 9 January 2018 diff hist +4 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 19:5819:58, 9 January 2018 diff hist +6 Low Salt Immune Complex Wash Buffer No edit summary current
• 19:5419:54, 9 January 2018 diff hist +355 N LiCl Immune Complex Wash Buffer Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | NP40 || 1% || 1.5mL || 1..." current
• 19:5419:54, 9 January 2018 diff hist +4 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 19:5319:53, 9 January 2018 diff hist +4 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 19:5219:52, 9 January 2018 diff hist −21 Low Salt Immune Complex Wash Buffer No edit summary
• 19:4819:48, 9 January 2018 diff hist +376 N Low Salt Immune Complex Wash Buffer Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | Example || Example || Ex..."
• 19:3019:30, 9 January 2018 diff hist −11 Chromatin Immunoprecipitation →Prior to starting this section:
• 19:3019:30, 9 January 2018 diff hist +3 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 19:2819:28, 9 January 2018 diff hist +6 Chromatin Immunoprecipitation →Reverse Crosslinks of Protein/DNA Complexes to Free DNA
• 19:2819:28, 9 January 2018 diff hist −1 Chromatin Immunoprecipitation →Prior to starting this section:
• 19:2719:27, 9 January 2018 diff hist +16 Chromatin Immunoprecipitation →Immunoprecipitation
• 19:2419:24, 9 January 2018 diff hist +6 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 19:2319:23, 9 January 2018 diff hist +19 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 19:1919:19, 9 January 2018 diff hist +58 Chromatin Immunoprecipitation →Immunoprecipitation
• 19:1719:17, 9 January 2018 diff hist −4 Chromatin Immunoprecipitation No edit summary
• 19:1419:14, 9 January 2018 diff hist −32 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 19:1119:11, 9 January 2018 diff hist +3,234 Chromatin Immunoprecipitation →Immunoprecipitation

5 January 2018

• 21:4321:43, 5 January 2018 diff hist +2 Preparation of Protein Lysates from Cells No edit summary current

15 December 2017

• 18:3318:33, 15 December 2017 diff hist −161 Preparation of Protein Lysates from Cells No edit summary
• 18:2818:28, 15 December 2017 diff hist +1,041 N Preparation of Protein Lysates from Cells Created page with "==Materials== *RIPA Buffer (see RIPA) or other Lysis buffer. Add protease inhibitors. *Cells (fresh or frozen) ==Protocol== #Cool centrifuge to 4C #If cells..."

24 October 2017

• 16:2016:20, 24 October 2017 diff hist 0 Submitting Plasmids for Sequencing No edit summary current
• 16:1916:19, 24 October 2017 diff hist +159 Submitting Plasmids for Sequencing No edit summary

19 October 2017

• 19:4119:41, 19 October 2017 diff hist +114 Cloning CRISPR-Cas Plasmids No edit summary current
• 19:3819:38, 19 October 2017 diff hist +32 Submitting Plasmids for Sequencing No edit summary
• 19:3519:35, 19 October 2017 diff hist +752 Submitting Plasmids for Sequencing No edit summary
• 19:2619:26, 19 October 2017 diff hist +975 N Submitting Plasmids for Sequencing Created page with "*Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul *These should be submitted in 1.5mL tubes *Go to https://client-seqcore.brcf.med.um..."

11 August 2017

• 15:5215:52, 11 August 2017 diff hist −8 Western Blotting →Protocol

8 August 2017

• 15:5215:52, 8 August 2017 diff hist +83 Bradford Assay →Protocol
• 15:5115:51, 8 August 2017 diff hist −4 Bradford Assay →Protocol