User contributions for Iharvey

21 March 2016

• 20:4320:43, 21 March 2016 diff hist −15 Chromatin Immunoprecipitation →Reverse cross-linking and recover ChIP DNA
• 20:4220:42, 21 March 2016 diff hist +3 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 20:3820:38, 21 March 2016 diff hist +32 Chromatin Immunoprecipitation →Buffers and Solutions Needed
• 20:3720:37, 21 March 2016 diff hist +6 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 20:3720:37, 21 March 2016 diff hist −4 Chromatin Immunoprecipitation →Incubate bead-antibody complex with fragmented, cross-linked chromatin
• 20:3720:37, 21 March 2016 diff hist +6 Chromatin Immunoprecipitation →Reverse cross-linking and recover ChIP DNA
• 20:3620:36, 21 March 2016 diff hist −25 Chromatin Immunoprecipitation →Analysis of Immunoprecipitated DNA
• 20:3620:36, 21 March 2016 diff hist +1,117 Chromatin Immunoprecipitation →Reverse cross-linking and recover ChIP DNA
• 20:2520:25, 21 March 2016 diff hist +184 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 20:1920:19, 21 March 2016 diff hist +76 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 20:1520:15, 21 March 2016 diff hist +22 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 20:1220:12, 21 March 2016 diff hist +22 Chromatin Immunoprecipitation →Buffers and Solutions Needed
• 20:0920:09, 21 March 2016 diff hist +12 Chromatin Immunoprecipitation →Incubate bead-antibody complex with fragmented, cross-linked chromatin
• 20:0020:00, 21 March 2016 diff hist −1 Chromatin Immunoprecipitation →Buffers and Solutions Needed
• 19:5919:59, 21 March 2016 diff hist +27 Chromatin Immunoprecipitation →Buffers and Solutions Needed

1 February 2016

• 22:4022:40, 1 February 2016 diff hist +51 Assessing isoproterenol-stimulated whole-body lipolysis in vivo →Experimental protocol

28 January 2016

• 18:1318:13, 28 January 2016 diff hist −1 Preparation of RNA Samples from Mouse Tissues →Protocol
• 18:1318:13, 28 January 2016 diff hist +40 Preparation of RNA Samples from Mouse Tissues →Protocol

26 January 2016

• 20:2820:28, 26 January 2016 diff hist 0 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 18:1718:17, 26 January 2016 diff hist +5 Chromatin Immunoprecipitation →Incubate bead-antibody complex with fragmented, cross-linked chromatin
• 18:1518:15, 26 January 2016 diff hist +294 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 18:0118:01, 26 January 2016 diff hist −1 Chromatin Immunoprecipitation →Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
• 18:0018:00, 26 January 2016 diff hist +73 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA

25 January 2016

• 17:1917:19, 25 January 2016 diff hist −23 RT-PCR primer design for ChIP No edit summary
• 17:1817:18, 25 January 2016 diff hist 0 RT-PCR primer design for ChIP No edit summary
• 17:1717:17, 25 January 2016 diff hist +6 RT-PCR primer design for ChIP No edit summary
• 17:1517:15, 25 January 2016 diff hist +1,524 N Locating ChIP-seq peaks from ENCODE Created page with "# Go to the UCSC website [https://genome.ucsc.edu/index.html] # Go to the ENCODE option on the left-hand side of the page # Click on the Explore ENCODE data at UCSC picture #..." current
• 16:4916:49, 25 January 2016 diff hist +38 Chromatin Immunoprecipitation →Analysis of Immunoprecipitated DNA
• 16:4416:44, 25 January 2016 diff hist +1,321 N RT-PCR primer design for ChIP Created page with "# Locate the potential gene regions you believe your protein is bound to (for glucocorticoid-induced ChIP-seq peaks refer to Locating ChIP-seq Peaks protocol). Make sure this..."

20 January 2016

• 18:5618:56, 20 January 2016 diff hist 0 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 18:5518:55, 20 January 2016 diff hist −1 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 18:0918:09, 20 January 2016 diff hist −12 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 18:0618:06, 20 January 2016 diff hist +15 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 16:5716:57, 20 January 2016 diff hist +1 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 16:5616:56, 20 January 2016 diff hist +4 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 16:5316:53, 20 January 2016 diff hist +69 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 16:3616:36, 20 January 2016 diff hist +581 Chromatin Immunoprecipitation →Immunoprecipitation
• 16:2716:27, 20 January 2016 diff hist +1,683 Chromatin Immunoprecipitation →Immunoprecipitation
• 16:1616:16, 20 January 2016 diff hist −1 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 16:1616:16, 20 January 2016 diff hist +1,060 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 16:0216:02, 20 January 2016 diff hist +866 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA

1 July 2015

• 20:2020:20, 1 July 2015 diff hist +558 QPCR →Plate Preparation

23 June 2015

• 19:4019:40, 23 June 2015 diff hist +19 Pyruvate Tolerance Test No edit summary
• 19:3819:38, 23 June 2015 diff hist +1,258 N Pyruvate Tolerance Test Created page with "'''Materials''' Glucometer - AccuChek Advantage Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2..."

29 August 2014

• 15:2615:26, 29 August 2014 diff hist +13 Oil red o stain for in vitro adipogenesis No edit summary
• 15:2515:25, 29 August 2014 diff hist +12 Oil red o stain for in vitro adipogenesis No edit summary
• 15:2415:24, 29 August 2014 diff hist +2 Oil red o stain for in vitro adipogenesis No edit summary
• 15:2315:23, 29 August 2014 diff hist +3,289 N Oil red o stain for in vitro adipogenesis Created page with "Oil red o stain for ''in vitro'' adipogenesis '''Materials''' •DPBS – Lonza catalog # 17-512 or equivalent •10% formalin, neutral buffered – VWR catalog # VW3239-4 o..."

5 August 2014

• 18:1218:12, 5 August 2014 diff hist −1 Triglyceride Assay from Tissue Culture Cells →Protocol current
• 18:1118:11, 5 August 2014 diff hist +446 Triglyceride Assay from Tissue Culture Cells →Protocol