Transfer Buffer: Difference between revisions
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copied recipe from saltiel lab buffers |
m →10X Transfer Buffer: Changed from 10L total to 4L total |
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{| border="1" | {| border="1" | ||
! Component || Weight per | ! Component || Weight per 4 L || Catalog Number | ||
|- | |- | ||
|Tris Base || | |Tris Base || 121.2 g || Sigma T6066-5KG | ||
|- | |- | ||
|Glycine || | |Glycine || 576.4 g || Sigma G7126-5KG | ||
|} | |} | ||
[[ Category:Buffer ]] | [[ Category:Buffer ]] | ||
[[ Category:Western Blotting ]] | [[ Category:Western Blotting ]] | ||
Latest revision as of 19:34, 11 July 2013
10X Transfer Buffer
To make 1X TBS, dilute 10X with water, and add 20% methanol. For example:
- 100 mL 10X Buffer
- 200 mL Methanol
- 700 mL Water
| Component | Weight per 4 L | Catalog Number |
|---|---|---|
| Tris Base | 121.2 g | Sigma T6066-5KG |
| Glycine | 576.4 g | Sigma G7126-5KG |