User contributions for Mpeloqu1

Results for Mpeloqu1 talk block log uploads logs

A user with 34 edits. Account created on 19 June 2013.

Jump to navigation Jump to search

This user is currently blocked. The latest block log entry is provided below for reference:

18:42, 26 January 2023 Davebridges talk contribs blocked Mpeloqu1 talk contribs with an expiration time of indefinite (account creation disabled)

14:1314:13, 1 April 2014 diff hist +491 N BDNF PCR Created page with "'''BDNF Primers''' ---- * oIMR0133 ---- ATG AAA GAA GTA AAC GTC CAC (Common) * oIMR0113 ---- CCA GCA GAA AGA GTA GAG GAG (WT Reverse) * oIMR01694 --- GGG AAC TTC CTG ACT AG..." current
14:0314:03, 1 April 2014 diff hist +13 Main Page Added BNDF

15:0315:03, 19 February 2014 diff hist −1 Rapamycin Injections No edit summary

15:0915:09, 1 November 2013 diff hist +990 N Paraffin Embedding of Tissue Samples Created page with " == Protocol == #After harvesting tissue from animal, place into pathology cassette and fix in 4% formaldehyde, 4% paraformaldehyde or 4% formalin O/N #After fixation, begin d..."
14:5014:50, 1 November 2013 diff hist +42 Main Page →Tissue Preparation

13:5613:56, 13 September 2013 diff hist 0 Fly Food Protocol →Cooking Insturctions current

17:3717:37, 3 September 2013 diff hist +7 Fly Food Protocol →Cooking Insturctions
17:3617:36, 3 September 2013 diff hist +2,037 N Fly Food Protocol Created page with " == Quantities == '''Summer''' #900mL - Corn Syrup #202.4g - Yeast #117.2g - Soy Flour #856g - Corn Meal #67.6g - Agar #15L - Water #65mL - Propionic Acid..."
16:2916:29, 3 September 2013 diff hist +23 Main Page →Fly Stocks

15:2315:23, 7 August 2013 diff hist +17 RIPA Buffer →RIPA Buffer (for 10mL lysis buffer)
15:2215:22, 7 August 2013 diff hist +32 RIPA Buffer →RIPA Buffer (for 10mL lysis buffer)
15:2115:21, 7 August 2013 diff hist 0 RIPA Buffer →RIPA Buffer (for 10mL lysis buffer)

16:0916:09, 6 August 2013 diff hist +1 Maintenance of 18C Fly Stocks →=Maintenance current
16:0916:09, 6 August 2013 diff hist +397 N Maintenance of 18C Fly Stocks Created page with "==Location== Drosophila stock vials can be found in the 18C cooler of the Reiter Laboratory ==Maintenance= *Drosophila stocks are to be flipped every 3 weeks *When flipping t..."
16:0416:04, 6 August 2013 diff hist 0 Main Page →Fly Stocks
16:0216:02, 6 August 2013 diff hist +360 N Maintanence of 18C Fly Stocks Created page with "==Location== Drosophila stocks can be found in the 18C cooler of the Reiter Lab ==Maintenance== *Drosophila stocks are to be "flipped" every 3 weeks *Always flip the oldest o..." current
15:5515:55, 6 August 2013 diff hist +51 Main Page →Mouse/Fly Protocols
15:5415:54, 6 August 2013 diff hist +4 Main Page →Mouse Protocols

14:2914:29, 1 August 2013 diff hist +102 Triglyceride Assay from Drosophila (German Method) →Protocol current

21:2621:26, 31 July 2013 diff hist +1,305 Triglyceride Assay from Cells and Tissues →Protocol
21:1221:12, 31 July 2013 diff hist +412 N Triglyceride Assay from Drosophila (German Method) Created page with "==Protocol== #Use 5 female or 8 Male flies for Assay #Homogenize flies (3 min @ 40Hz) in 1mL of .05% Tween (from Tween-20 stock) #Heat samples in 70C waterbath for 5 minutes #..."
21:1121:11, 31 July 2013 diff hist −1,406 Triglyceride Assay from Cells and Tissues →Protocol: Creation of Protocol
21:0521:05, 31 July 2013 diff hist +56 m Main Page →Protein Purification

21:2721:27, 26 July 2013 diff hist +13 m Preparation of Protein Lysates from Mouse Tissues →Protocol
21:2321:23, 26 July 2013 diff hist +266 m Bradford Assay →Protocol: Added Protein Lysate
13:5513:55, 26 July 2013 diff hist +39 PCR Analysis of Tail DNA →Protocol

15:0015:00, 25 July 2013 diff hist +247 m PCR Analysis of Tail DNA →Protocol: Master Mix

19:3919:39, 11 July 2013 diff hist +133 m Western Blotting →Protocol: LiCor Addition
19:3419:34, 11 July 2013 diff hist +2 m Transfer Buffer →10X Transfer Buffer: Changed from 10L total to 4L total current
19:3319:33, 11 July 2013 diff hist −6 m Western Blotting →Protocol: Changed wash times

17:1017:10, 28 June 2013 diff hist 0 m Triglyceride Assay from Cells and Tissues →Protocol: Volume of butanol mixture

17:1817:18, 26 June 2013 diff hist +31 m Preparation of Tail Samples (for Genotyping) →Protocol: Proteinase K

15:2915:29, 19 June 2013 diff hist −55 m Triglyceride Assay from Cells and Tissues Deletion of unnecessary information (1/10 Glycerol Dilution)
15:2415:24, 19 June 2013 diff hist −466 Triglyceride Assay from Cells and Tissues updated volumes and lysis procedure

Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php/Special:Contributions/Mpeloqu1"