Dilution Formula: Difference between revisions

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Created page with 'C1V1=C2V2 C1=measured absorption V1=unknown volume to be added to final volume V2=desired volume to re-suspend V1 in (usually 4mL) C2=<math>OD/2^x</math> x=amount of times ce...'
 
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C1V1=C2V2
C1V1=C2V2


C1=measured absorption  
*C1=measured absorption  
V1=unknown volume to be added to final volume  
*V1=unknown volume to be added to final volume  
V2=desired volume to re-suspend V1 in (usually 4mL)  
*V2=desired volume to re-suspend V1 in (usually 4mL)  
C2=<math>OD/2^x</math>
*C2=OD/2^x
x=amount of times cells will double
 
^find this by dividing the number of hours the cells will be growing by how long one doubling period is
*x=amount of times cells will double
*find this by dividing the number of hours the cells will be growing by how long one doubling period is

Latest revision as of 13:46, 29 June 2010

C1V1=C2V2

  • C1=measured absorption
  • V1=unknown volume to be added to final volume
  • V2=desired volume to re-suspend V1 in (usually 4mL)
  • C2=OD/2^x
  • x=amount of times cells will double
  • find this by dividing the number of hours the cells will be growing by how long one doubling period is