User contributions for Kalefish

12 August 2010

• 19:5719:57, 12 August 2010 diff hist +98 CPY Assay No edit summary current

10 August 2010

• 16:2116:21, 10 August 2010 diff hist +9 GoTaq PCR Genotyping No edit summary
• 16:2116:21, 10 August 2010 diff hist +862 N GoTaq PCR Genotyping Created page with '==Materials(100uM)== *GoTaq Master Mix *Primer Mix: Add 10uL of forward and reverse primer, 20uL total, into 1mL of dH20. *Reaction Mixture: 25uL GoTaq Mix, 10uL of Primer Mix, ...'

8 July 2010

• 14:1514:15, 8 July 2010 diff hist −146 CPY Assay →Protocol

7 July 2010

• 16:3816:38, 7 July 2010 diff hist +41 CPY Assay →Protocol
• 16:3516:35, 7 July 2010 diff hist +554 N CPY Assay Created page with 'Carboxypeptidase Secretion Assay == Protocol == *Revive Yeast Cells (2-3) days *Pick one colony and make a 1/2 inch x 1/2 inch square patch of yeast. (Does this have to be sele...'

29 June 2010

• 13:4613:46, 29 June 2010 diff hist −7 Dilution Formula No edit summary current
• 13:4513:45, 29 June 2010 diff hist +297 N Dilution Formula Created page with 'C1V1=C2V2 C1=measured absorption V1=unknown volume to be added to final volume V2=desired volume to re-suspend V1 in (usually 4mL) C2=<math>OD/2^x</math> x=amount of times ce...'

28 June 2010

• 18:4718:47, 28 June 2010 diff hist +901 GST-EEA1 Pulldown from Yeast No edit summary