Triglyceride Assay from Drosophila (German Method): Difference between revisions
Jump to navigation
Jump to search
Created page with "==Protocol== #Use 5 female or 8 Male flies for Assay #Homogenize flies (3 min @ 40Hz) in 1mL of .05% Tween (from Tween-20 stock) #Heat samples in 70C waterbath for 5 minutes #..." |
|||
| Line 9: | Line 9: | ||
#Incubate plate @ 37C for 5 mins | #Incubate plate @ 37C for 5 mins | ||
#Measure 540nm absorbance | #Measure 540nm absorbance | ||
==Publication of Method== | |||
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023796 | |||
Latest revision as of 14:29, 1 August 2013
Protocol
- Use 5 female or 8 Male flies for Assay
- Homogenize flies (3 min @ 40Hz) in 1mL of .05% Tween (from Tween-20 stock)
- Heat samples in 70C waterbath for 5 minutes
- Spin samples @ 5000G for 1 minute
- Transfer 500ul of supernatent in new microfuge tube
- Spin @ 14000G for 3 minutes
- Add 50ul of sample to 200ul of TG solution in a 96 well plate
- Incubate plate @ 37C for 5 mins
- Measure 540nm absorbance
Publication of Method
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023796