RIPA Buffer

From Bridges Lab Protocols

Revision as of 15:28, 21 December 2009 by Davebrid (talk | contribs) (changed vanadate concentration and volume)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Jump to navigation Jump to search

RIPA Buffer (for 10mL lysis buffer)

	Final Concentration	per 10 mL	Stock	Location
Tris pH7.4	50mM	500uL	1M	Shelf
Na Deoxycholate	0.25%	250uL	10%	Shelf
NP-40	1%	1mL	10%	Tingting
NaCl	150mM	375uL	4M	Shelf
EDTA	1mM	20uL	0.5M	Shelf
NaVO3	100uM	10uL	100mM	Biochemical Stocks (-20)
NaF	5mM	100uL	500mM	Shelf
NaPPi	25 mM	1 mL	250mM	Shelf
Protease Inhibitors		1 mini tablet		4C

Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php?title=RIPA_Buffer&oldid=442"

Buffer