From Bridges Lab Protocols
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Cholesterol Assay
Materials
- Reagent
- Cholesterol Standard
Protocol
- Make 10X stock dilution: Add 50ɥL Cholesterol Standard 450ɥL water to a 1.5mL tube
- Pipette 100ɥL of Reagent to 22 wells
- Add 2-10ɥL of 10X stock dilution to wells in columns 2-6 in increments of 2ɥL. Each column will have 2 replicates per volume (1 replicate per row).
- Repeat in columns 7-11 with 2-10ɥL of Cholesterol Standard (1X dilution)
- Allow reaction to proceed for 15 minutes
- Read absorbance