User contributions for Davebrid

7 March 2022

• 15:1915:19, 7 March 2022 diff hist +537 Insulin Tolerance Test added abstract

7 April 2020

• 20:0220:02, 7 April 2020 diff hist +306 Preparation of Chloroquine Stocks updated due to uptick in COVID search results for chloroquine current

5 February 2020

• 19:1619:16, 5 February 2020 diff hist +118 Writing a Paper and Uploading Code to Github Repository →Initial Steps: Updated initial sections for manuscript current

11 November 2019

• 17:2517:25, 11 November 2019 diff hist +1 Generation of Figures in Illustrator No edit summary current

6 November 2019

• 15:0115:01, 6 November 2019 diff hist +1,843 Writing a Paper and Uploading Code to Github Repository Added more details about writing discussion and introduction sections
• 14:5314:53, 6 November 2019 diff hist +2,862 Writing a Paper and Uploading Code to Github Repository Wrote about uploading data
• 14:3814:38, 6 November 2019 diff hist +2,835 N Writing a Paper and Uploading Code to Github Repository Wrote initial page, included instructions for github
• 14:1914:19, 6 November 2019 diff hist +695 Generation of Figures in Illustrator Clarified instructions for making figures

18 April 2019

• 13:1613:16, 18 April 2019 diff hist +857 Sequence Alignment for Phylogenetic Analysis Added details about BLAST search current
• 13:0713:07, 18 April 2019 diff hist +1,167 Sequence Alignment for Phylogenetic Analysis Added info about FASTA code
• 12:5812:58, 18 April 2019 diff hist +322 Sequence Alignment for Phylogenetic Analysis Added PhyloBayes information
• 12:2412:24, 18 April 2019 diff hist +329 N Sequence Alignment for Phylogenetic Analysis Wrote initial page

5 September 2018

• 13:3113:31, 5 September 2018 diff hist +2,667 N Using GSEA for Gene Set Analyses of Transcriptional Data Wrote initial protocol for GSEA current

31 July 2018

• 14:5514:55, 31 July 2018 diff hist +458 Glucose Uptake Assay →Materials: updated with catalog numbers current

25 April 2018

• 18:4218:42, 25 April 2018 diff hist +87 Cre PCR added primers current

18 March 2018

• 15:0415:04, 18 March 2018 diff hist +15 Differentiation of 3T3-L1 Cells →Materials: updated concentration of IBMX

25 January 2018

• 21:5521:55, 25 January 2018 diff hist 0 Phosphate Buffered Saline No edit summary current
• 21:5421:54, 25 January 2018 diff hist −1 Phosphate Buffered Saline →Recipe
• 21:3121:31, 25 January 2018 diff hist +267 N Phosphate Buffered Saline Created page with "==Recipe== * Start with 800 ml of distilled water: * Add 8 g of NaCl. * Add 0.2 g of KCl. * Add 1.44 g of Na₂HPO₄. * Add 0.24 g of KH₂PO<sub>4..."

19 December 2017

• 16:1516:15, 19 December 2017 diff hist +1,385 N PEI Mediated Plasmid Transfection Wrote initial protocol for PEI transfections current

12 December 2017

• 15:1015:10, 12 December 2017 diff hist +120 N Category:CRISPR Wrote cover page for CRISPR current

11 December 2017

• 02:1102:11, 11 December 2017 diff hist +11,397 N General Statistics copied from markdown converted mediawiki

8 December 2017

• 16:2016:20, 8 December 2017 diff hist +10 Production of LentiCRISPR Viruses Removed table of contents current
• 16:1916:19, 8 December 2017 diff hist +237 Production of LentiCRISPR Viruses Made new sections and added part about chloroquine
• 16:1616:16, 8 December 2017 diff hist +327 Preparation of PEI Stocks Added notes about testing transfection efficiency of PEI batches. current
• 16:1316:13, 8 December 2017 diff hist +147 Production of LentiCRISPR Viruses Added categories
• 16:1216:12, 8 December 2017 diff hist +1,998 N Production of LentiCRISPR Viruses Wrote initial protocol for virus production.
• 15:4715:47, 8 December 2017 diff hist +437 N Preparation of Chloroquine Stocks Wrote instructions for making chloroquine
• 15:4215:42, 8 December 2017 diff hist +147 m Preparation of PEI Stocks Added categories
• 15:4115:41, 8 December 2017 diff hist +414 N Preparation of PEI Stocks wrote instructions for making PEI stocks

16 October 2017

• 23:1923:19, 16 October 2017 diff hist +126 Measuring Fasting Insulin Updated with product details

4 October 2017

• 15:0515:05, 4 October 2017 diff hist +99 m Thawing Culture Cells Formatting changes current

2 August 2017

• 14:3214:32, 2 August 2017 diff hist +67 N Category:Media Created page with "This is for preparation of a variety of media for bacteria or yeast" current
• 14:3114:31, 2 August 2017 diff hist −1 Seahorse XF Media changed category tag current
• 14:2914:29, 2 August 2017 diff hist −1 Preparation of Orthovanadate Stocks changed category tag current
• 14:2814:28, 2 August 2017 diff hist −1 m ACSF Media changed category tag current
• 14:2714:27, 2 August 2017 diff hist −1 m 50X TAE Buffer →Reagents: changed category tag current
• 14:2714:27, 2 August 2017 diff hist +894 N Using the pH Meter wrote initial page current

31 July 2017

• 12:2812:28, 31 July 2017 diff hist +10 Seahorse - Mitochondrial Stress Test removed table of contents

20 July 2017

• 21:0421:04, 20 July 2017 diff hist +1,232 N BHB Assay wrote initial page for total ketone assay current

6 June 2017

• 12:3612:36, 6 June 2017 diff hist +2,097 N Isolation of Single Fibers for Seahorse Assays wrote initial protocol

29 May 2017

• 15:4215:42, 29 May 2017 diff hist +62 N Category:Lab Safety Created page with "This page lists all protocols and SOP's related to lab safety." current
• 15:4115:41, 29 May 2017 diff hist +2,075 N SOP - BVT.2733 copied OSEH findings into SOP

22 May 2017

• 20:0820:08, 22 May 2017 diff hist +73 N Category:Seahorse Created page with "These are the protocols and buffers we use with the Seahorse bioanalyser." current
• 20:0620:06, 22 May 2017 diff hist +2,216 Seahorse - Mitochondrial Stress Test Wrote initial page for seahorse assay

17 May 2017

• 14:5914:59, 17 May 2017 diff hist +177 Data Management Added link to Rmarkdown template

16 May 2017

• 19:3319:33, 16 May 2017 diff hist +63 N Seahorse - Mitochondrial Stress Test created placeholder page
• 13:3213:32, 16 May 2017 diff hist +7,747 N Data Management copied from pandoc generated mw file

27 April 2017

• 16:3916:39, 27 April 2017 diff hist −4 m Pyruvate Tolerance Test fixed typo
• 16:3516:35, 27 April 2017 diff hist +12 Pyruvate Tolerance Test Updated layout of the protocol