User contributions for Davebrid

18 March 2018

• 15:0415:04, 18 March 2018 diff hist +15 Differentiation of 3T3-L1 Cells →Materials: updated concentration of IBMX

25 January 2018

• 21:5521:55, 25 January 2018 diff hist 0 Phosphate Buffered Saline No edit summary current
• 21:5421:54, 25 January 2018 diff hist −1 Phosphate Buffered Saline →Recipe
• 21:3121:31, 25 January 2018 diff hist +267 N Phosphate Buffered Saline Created page with "==Recipe== * Start with 800 ml of distilled water: * Add 8 g of NaCl. * Add 0.2 g of KCl. * Add 1.44 g of Na₂HPO₄. * Add 0.24 g of KH₂PO<sub>4..."

19 December 2017

• 16:1516:15, 19 December 2017 diff hist +1,385 N PEI Mediated Plasmid Transfection Wrote initial protocol for PEI transfections current

12 December 2017

• 15:1015:10, 12 December 2017 diff hist +120 N Category:CRISPR Wrote cover page for CRISPR current

11 December 2017

• 02:1102:11, 11 December 2017 diff hist +11,397 N General Statistics copied from markdown converted mediawiki

8 December 2017

• 16:2016:20, 8 December 2017 diff hist +10 Production of LentiCRISPR Viruses Removed table of contents current
• 16:1916:19, 8 December 2017 diff hist +237 Production of LentiCRISPR Viruses Made new sections and added part about chloroquine
• 16:1616:16, 8 December 2017 diff hist +327 Preparation of PEI Stocks Added notes about testing transfection efficiency of PEI batches. current
• 16:1316:13, 8 December 2017 diff hist +147 Production of LentiCRISPR Viruses Added categories
• 16:1216:12, 8 December 2017 diff hist +1,998 N Production of LentiCRISPR Viruses Wrote initial protocol for virus production.
• 15:4715:47, 8 December 2017 diff hist +437 N Preparation of Chloroquine Stocks Wrote instructions for making chloroquine
• 15:4215:42, 8 December 2017 diff hist +147 m Preparation of PEI Stocks Added categories
• 15:4115:41, 8 December 2017 diff hist +414 N Preparation of PEI Stocks wrote instructions for making PEI stocks

16 October 2017

• 23:1923:19, 16 October 2017 diff hist +126 Measuring Fasting Insulin Updated with product details

4 October 2017

• 15:0515:05, 4 October 2017 diff hist +99 m Thawing Culture Cells Formatting changes current

2 August 2017

• 14:3214:32, 2 August 2017 diff hist +67 N Category:Media Created page with "This is for preparation of a variety of media for bacteria or yeast" current
• 14:3114:31, 2 August 2017 diff hist −1 Seahorse XF Media changed category tag current
• 14:2914:29, 2 August 2017 diff hist −1 Preparation of Orthovanadate Stocks changed category tag current
• 14:2814:28, 2 August 2017 diff hist −1 m ACSF Media changed category tag current
• 14:2714:27, 2 August 2017 diff hist −1 m 50X TAE Buffer →Reagents: changed category tag current
• 14:2714:27, 2 August 2017 diff hist +894 N Using the pH Meter wrote initial page current

31 July 2017

• 12:2812:28, 31 July 2017 diff hist +10 Seahorse - Mitochondrial Stress Test removed table of contents

20 July 2017

• 21:0421:04, 20 July 2017 diff hist +1,232 N BHB Assay wrote initial page for total ketone assay current

6 June 2017

• 12:3612:36, 6 June 2017 diff hist +2,097 N Isolation of Single Fibers for Seahorse Assays wrote initial protocol

29 May 2017

• 15:4215:42, 29 May 2017 diff hist +62 N Category:Lab Safety Created page with "This page lists all protocols and SOP's related to lab safety." current
• 15:4115:41, 29 May 2017 diff hist +2,075 N SOP - BVT.2733 copied OSEH findings into SOP

22 May 2017

• 20:0820:08, 22 May 2017 diff hist +73 N Category:Seahorse Created page with "These are the protocols and buffers we use with the Seahorse bioanalyser." current
• 20:0620:06, 22 May 2017 diff hist +2,216 Seahorse - Mitochondrial Stress Test Wrote initial page for seahorse assay

17 May 2017

• 14:5914:59, 17 May 2017 diff hist +177 Data Management Added link to Rmarkdown template

16 May 2017

• 19:3319:33, 16 May 2017 diff hist +63 N Seahorse - Mitochondrial Stress Test created placeholder page
• 13:3213:32, 16 May 2017 diff hist +7,747 N Data Management copied from pandoc generated mw file

27 April 2017

• 16:3916:39, 27 April 2017 diff hist −4 m Pyruvate Tolerance Test fixed typo
• 16:3516:35, 27 April 2017 diff hist +12 Pyruvate Tolerance Test Updated layout of the protocol
• 13:2113:21, 27 April 2017 diff hist +105 ACSF Media Updated KHPO4 concentration and fixed a typo
• 12:4212:42, 27 April 2017 diff hist +59 N Category:Buffers Created page with "This page lists recipes for buffers used in various assays." current
• 12:4112:41, 27 April 2017 diff hist +224 ACSF Media Added categories
• 12:4112:41, 27 April 2017 diff hist +165 Seahorse XF Media added categories
• 12:3912:39, 27 April 2017 diff hist +965 N ACSF Media Wrote initial table for buffers

28 March 2017

• 11:5611:56, 28 March 2017 diff hist +1,400 Adipose Tissue Nuclear Isolation Updated protocol with more details current

27 March 2017

• 20:4120:41, 27 March 2017 diff hist +885 N Adipose Tissue Nuclear Isolation copied initial protocol from Kang

10 March 2017

• 14:0714:07, 10 March 2017 diff hist +332 Seahorse XF Media updated stock concentrations

1 March 2017

• 15:2415:24, 1 March 2017 diff hist +328 Quantification of miRNA by SYBR Green qPCR updated with some clarifications current

27 February 2017

• 14:3014:30, 27 February 2017 diff hist 0 m Luciferase Assay Changed catalog number current
• 14:3014:30, 27 February 2017 diff hist −115 Luciferase Assay No edit summary
• 14:1814:18, 27 February 2017 diff hist +1,877 N Talk:Luciferase Assay copied older protocol over to talk page current

22 February 2017

• 21:0721:07, 22 February 2017 diff hist +1,087 N Seahorse XF Media Wrote initial page
• 20:1620:16, 22 February 2017 diff hist −174 QPCR Updated with smaller sample volumes

3 February 2017

• 13:3613:36, 3 February 2017 diff hist −5 Preparing and Sending Tissues to Histology Core Fixed broken link current