User contributions for Davebridges

19 December 2017

• 16:1516:15, 19 December 2017 diff hist +1,385 N PEI Mediated Plasmid Transfection Wrote initial protocol for PEI transfections current

12 December 2017

• 15:1015:10, 12 December 2017 diff hist +120 N Category:CRISPR Wrote cover page for CRISPR current

11 December 2017

• 02:1102:11, 11 December 2017 diff hist +11,397 N General Statistics copied from markdown converted mediawiki

8 December 2017

• 16:2016:20, 8 December 2017 diff hist +10 Production of LentiCRISPR Viruses Removed table of contents current
• 16:1916:19, 8 December 2017 diff hist +237 Production of LentiCRISPR Viruses Made new sections and added part about chloroquine
• 16:1616:16, 8 December 2017 diff hist +327 Preparation of PEI Stocks Added notes about testing transfection efficiency of PEI batches. current
• 16:1316:13, 8 December 2017 diff hist +147 Production of LentiCRISPR Viruses Added categories
• 16:1216:12, 8 December 2017 diff hist +1,998 N Production of LentiCRISPR Viruses Wrote initial protocol for virus production.
• 15:4715:47, 8 December 2017 diff hist +437 N Preparation of Chloroquine Stocks Wrote instructions for making chloroquine
• 15:4215:42, 8 December 2017 diff hist +147 m Preparation of PEI Stocks Added categories
• 15:4115:41, 8 December 2017 diff hist +414 N Preparation of PEI Stocks wrote instructions for making PEI stocks

16 October 2017

• 23:1923:19, 16 October 2017 diff hist +126 Measuring Fasting Insulin Updated with product details

4 October 2017

• 15:0515:05, 4 October 2017 diff hist +99 m Thawing Culture Cells Formatting changes current

2 August 2017

• 14:3214:32, 2 August 2017 diff hist +67 N Category:Media Created page with "This is for preparation of a variety of media for bacteria or yeast" current
• 14:3114:31, 2 August 2017 diff hist −1 Seahorse XF Media changed category tag current
• 14:2914:29, 2 August 2017 diff hist −1 Preparation of Orthovanadate Stocks changed category tag current
• 14:2814:28, 2 August 2017 diff hist −1 m ACSF Media changed category tag current
• 14:2714:27, 2 August 2017 diff hist −1 m 50X TAE Buffer →Reagents: changed category tag current
• 14:2714:27, 2 August 2017 diff hist +894 N Using the pH Meter wrote initial page current

31 July 2017

• 12:2812:28, 31 July 2017 diff hist +10 Seahorse - Mitochondrial Stress Test removed table of contents current

20 July 2017

• 21:0421:04, 20 July 2017 diff hist +1,232 N BHB Assay wrote initial page for total ketone assay current

6 June 2017

• 12:3612:36, 6 June 2017 diff hist +2,097 N Isolation of Single Fibers for Seahorse Assays wrote initial protocol

29 May 2017

• 15:4215:42, 29 May 2017 diff hist +62 N Category:Lab Safety Created page with "This page lists all protocols and SOP's related to lab safety." current
• 15:4115:41, 29 May 2017 diff hist +2,075 N SOP - BVT.2733 copied OSEH findings into SOP

22 May 2017

• 20:0820:08, 22 May 2017 diff hist +73 N Category:Seahorse Created page with "These are the protocols and buffers we use with the Seahorse bioanalyser." current
• 20:0620:06, 22 May 2017 diff hist +2,216 Seahorse - Mitochondrial Stress Test Wrote initial page for seahorse assay

17 May 2017

• 14:5914:59, 17 May 2017 diff hist +177 Data Management Added link to Rmarkdown template

16 May 2017

• 19:3319:33, 16 May 2017 diff hist +63 N Seahorse - Mitochondrial Stress Test created placeholder page
• 13:3213:32, 16 May 2017 diff hist +7,747 N Data Management copied from pandoc generated mw file

27 April 2017

• 16:3916:39, 27 April 2017 diff hist −4 m Pyruvate Tolerance Test fixed typo
• 16:3516:35, 27 April 2017 diff hist +12 Pyruvate Tolerance Test Updated layout of the protocol
• 13:2113:21, 27 April 2017 diff hist +105 ACSF Media Updated KHPO4 concentration and fixed a typo
• 12:4212:42, 27 April 2017 diff hist +59 N Category:Buffers Created page with "This page lists recipes for buffers used in various assays." current
• 12:4112:41, 27 April 2017 diff hist +224 ACSF Media Added categories
• 12:4112:41, 27 April 2017 diff hist +165 Seahorse XF Media added categories
• 12:3912:39, 27 April 2017 diff hist +965 N ACSF Media Wrote initial table for buffers

28 March 2017

• 11:5611:56, 28 March 2017 diff hist +1,400 Adipose Tissue Nuclear Isolation Updated protocol with more details current

27 March 2017

• 20:4120:41, 27 March 2017 diff hist +885 N Adipose Tissue Nuclear Isolation copied initial protocol from Kang

10 March 2017

• 14:0714:07, 10 March 2017 diff hist +332 Seahorse XF Media updated stock concentrations

1 March 2017

• 15:2415:24, 1 March 2017 diff hist +328 Quantification of miRNA by SYBR Green qPCR updated with some clarifications current

27 February 2017

• 14:3014:30, 27 February 2017 diff hist 0 m Luciferase Assay Changed catalog number current
• 14:3014:30, 27 February 2017 diff hist −115 Luciferase Assay No edit summary
• 14:1814:18, 27 February 2017 diff hist +1,877 N Talk:Luciferase Assay copied older protocol over to talk page current

22 February 2017

• 21:0721:07, 22 February 2017 diff hist +1,087 N Seahorse XF Media Wrote initial page
• 20:1620:16, 22 February 2017 diff hist −174 QPCR Updated with smaller sample volumes

3 February 2017

• 13:3613:36, 3 February 2017 diff hist −5 Preparing and Sending Tissues to Histology Core Fixed broken link current
• 13:3513:35, 3 February 2017 diff hist +80 Preparing and Sending Tissues to Histology Core Added link to histology form
• 13:3113:31, 3 February 2017 diff hist +138 N File:UMCCC Histology Request Form.pdf This form is for submitting files to the UMCCC Histology Core. Enclose it in the pouch of the bag with your samples when dropping it off. current

29 January 2017

• 19:3519:35, 29 January 2017 diff hist +1,465 N Set up qPCR Run on Thermo Cloud and QuantStudio Wrote information for uploading plate to thermo cloud.
• 18:5918:59, 29 January 2017 diff hist +133 Set up qPCR Run on Roche LightCycler added categories current