User contributions for Davebrid

27 April 2017

• 13:2113:21, 27 April 2017 diff hist +105 ACSF Media Updated KHPO4 concentration and fixed a typo
• 12:4212:42, 27 April 2017 diff hist +59 N Category:Buffers Created page with "This page lists recipes for buffers used in various assays." current
• 12:4112:41, 27 April 2017 diff hist +224 ACSF Media Added categories
• 12:4112:41, 27 April 2017 diff hist +165 Seahorse XF Media added categories
• 12:3912:39, 27 April 2017 diff hist +965 N ACSF Media Wrote initial table for buffers

28 March 2017

• 11:5611:56, 28 March 2017 diff hist +1,400 Adipose Tissue Nuclear Isolation Updated protocol with more details current

27 March 2017

• 20:4120:41, 27 March 2017 diff hist +885 N Adipose Tissue Nuclear Isolation copied initial protocol from Kang

10 March 2017

• 14:0714:07, 10 March 2017 diff hist +332 Seahorse XF Media updated stock concentrations

1 March 2017

• 15:2415:24, 1 March 2017 diff hist +328 Quantification of miRNA by SYBR Green qPCR updated with some clarifications current

27 February 2017

• 14:3014:30, 27 February 2017 diff hist 0 m Luciferase Assay Changed catalog number current
• 14:3014:30, 27 February 2017 diff hist −115 Luciferase Assay No edit summary
• 14:1814:18, 27 February 2017 diff hist +1,877 N Talk:Luciferase Assay copied older protocol over to talk page current

22 February 2017

• 21:0721:07, 22 February 2017 diff hist +1,087 N Seahorse XF Media Wrote initial page
• 20:1620:16, 22 February 2017 diff hist −174 QPCR Updated with smaller sample volumes

3 February 2017

• 13:3613:36, 3 February 2017 diff hist −5 Preparing and Sending Tissues to Histology Core Fixed broken link current
• 13:3513:35, 3 February 2017 diff hist +80 Preparing and Sending Tissues to Histology Core Added link to histology form
• 13:3113:31, 3 February 2017 diff hist +138 N File:UMCCC Histology Request Form.pdf This form is for submitting files to the UMCCC Histology Core. Enclose it in the pouch of the bag with your samples when dropping it off. current

29 January 2017

• 19:3519:35, 29 January 2017 diff hist +1,465 N Set up qPCR Run on Thermo Cloud and QuantStudio Wrote information for uploading plate to thermo cloud.
• 18:5918:59, 29 January 2017 diff hist +133 Set up qPCR Run on Roche LightCycler added categories current
• 18:5918:59, 29 January 2017 diff hist +46 m QPCR added categories
• 18:5818:58, 29 January 2017 diff hist +554 N Set up qPCR Run on Roche LightCycler Copied instructions from qPCR page
• 18:5718:57, 29 January 2017 diff hist −379 QPCR →Real Time qPCR: Added links to separate pages for setting up qPCR runs

10 January 2017

• 19:0119:01, 10 January 2017 diff hist +995 Quantification of miRNA by SYBR Green qPCR Added RNA tailing
• 18:3818:38, 10 January 2017 diff hist +18 Quantification of miRNA by SYBR Green qPCR Added link to Pfeffer article

2 January 2017

• 15:4015:40, 2 January 2017 diff hist +228 Using the echoMRI Updated after Kari's suggestions current

9 December 2016

• 15:3415:34, 9 December 2016 diff hist +214 Culturing and Differentiating C2C12 Cells updated with more media details current

6 December 2016

• 21:1521:15, 6 December 2016 diff hist +328 QPCR →Real Time qPCR: updated catalog numbers
• 21:1021:10, 6 December 2016 diff hist +95 m Preparation of RNA Samples from Mouse Tissues Added SOP's to RNA protocol
• 21:0821:08, 6 December 2016 diff hist +97 m SOP - Phenol signed SOP - DB

15 November 2016

• 13:0613:06, 15 November 2016 diff hist +1,294 N Using the echoMRI wrote initial page

8 November 2016

• 14:5314:53, 8 November 2016 diff hist +97 m SOP - Autoclave →Certification: DB signed
• 14:5114:51, 8 November 2016 diff hist +95 SOP - Flammables and Combustibles →Certification: DB signed
• 14:5014:50, 8 November 2016 diff hist +97 m SOP - Bunsen Burners →Training of Personnel: DB signed

18 September 2016

• 12:3312:33, 18 September 2016 diff hist +4 Bridges Lab Protocols:About Updated link to website. current
• 12:3012:30, 18 September 2016 diff hist +232 N Bridges Lab Protocols:General disclaimer Wrote general disclaimer current

13 September 2016

• 14:4414:44, 13 September 2016 diff hist +525 Spill Response Procedures for Infectious agents and Recombinant DNA Added certification section
• 13:4913:49, 13 September 2016 diff hist −1 m Spill Response Procedures for Infectious agents and Recombinant DNA fixed spacing problem
• 13:4813:48, 13 September 2016 diff hist +5,010 N Spill Response Procedures for Infectious agents and Recombinant DNA Wrote initial spill SOP

21 August 2016

• 14:2514:25, 21 August 2016 diff hist +42 m Chromatin Immunoprecipitation Added category
• 14:2514:25, 21 August 2016 diff hist +105 N Category:Transcription wrote initial page current
• 14:2414:24, 21 August 2016 diff hist +93 m RT-PCR primer design for ChIP added categories current
• 14:2314:23, 21 August 2016 diff hist +54 m Immunoprecipitation added categories current

15 August 2016

• 18:5518:55, 15 August 2016 diff hist −18 SOP - Chloroform Fixed formatting
• 14:4214:42, 15 August 2016 diff hist +9,500 N SOP - Chloroform Wrote initial page
• 12:1012:10, 15 August 2016 diff hist 0 Main Page →Lab Safety current
• 12:1012:10, 15 August 2016 diff hist +223 Main Page Added SOP's to front page

9 August 2016

• 14:0214:02, 9 August 2016 diff hist 0 File:SHROB Genotyping.png Davebridges uploaded a new version of File:SHROB Genotyping.png current
• 14:0114:01, 9 August 2016 diff hist +150 SHROB Genotyping Made older method collapsable
• 13:5713:57, 9 August 2016 diff hist +29 SHROB Genotyping Added schematic
• 13:5613:56, 9 August 2016 diff hist +75 N File:SHROB Genotyping.png This schematic shows the fragments that are generated with SHROB Genotyping