User contributions for Davebrid

9 August 2016

• 13:4913:49, 9 August 2016 diff hist +855 SHROB Genotyping Added new SHROB Genotyping protocol

1 August 2016

• 14:0114:01, 1 August 2016 diff hist +53 N Category:Buffer Created page with "This page lists buffers used for a variety of assays." current

26 July 2016

• 20:5520:55, 26 July 2016 diff hist +27 m SOP - Bleach added category
• 20:4820:48, 26 July 2016 diff hist +573 Safety and Animal Training Added link to SOP's
• 20:4020:40, 26 July 2016 diff hist +169 SOP - Bleach →Certification: signed SOP
• 20:3620:36, 26 July 2016 diff hist +54 N Category:SOP Created page with "This page lists all Lab Standard Operating Procedures." current
• 20:3520:35, 26 July 2016 diff hist +11,713 N SOP - Bleach copied initial page from Michigan OSEH site

24 July 2016

• 02:2302:23, 24 July 2016 diff hist +4 Main Page Changed dept website link

21 July 2016

• 20:1520:15, 21 July 2016 diff hist +471 N Safety and Animal Training wrote initial page

24 June 2016

• 13:2213:22, 24 June 2016 diff hist −47 m Insulin Tolerance Test →Materials (Bring Downstairs): removed link
• 13:2213:22, 24 June 2016 diff hist +2 m Insulin Tolerance Test changed section link
• 13:2113:21, 24 June 2016 diff hist +2,181 Insulin Tolerance Test wrote section regarding fat mass considerations

5 February 2016

• 22:3922:39, 5 February 2016 diff hist +56 N Category:Animal Studies Created page with "This is for protocols associated with animal experiments" current

3 February 2016

• 20:3520:35, 3 February 2016 diff hist +3,917 N PAS Staining copied protocol

1 February 2016

• 23:4123:41, 1 February 2016 diff hist +66 Chromatin Immunoprecipitation →Analysis of Immunoprecipitated DNA: added dilution to ChIP

27 January 2016

• 20:5720:57, 27 January 2016 diff hist +61 RIPA Buffer →RIPA Buffer (for 10mL lysis buffer): added link to orthovanadate protocol current
• 20:5720:57, 27 January 2016 diff hist +61 Preparing Cell Lysates →RIPA Buffer (for 10mL lysis buffer): added link to orthovanadate protocol current
• 20:5620:56, 27 January 2016 diff hist +831 N Preparation of Orthovanadate Stocks Wrote initial page
• 17:3517:35, 27 January 2016 diff hist +97 m Assessing isoproterenol-stimulated whole-body lipolysis in vivo Added categories

20 January 2016

• 21:1021:10, 20 January 2016 diff hist +23 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA: specified tube for sonicaiton
• 18:3918:39, 20 January 2016 diff hist −2 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
• 18:3618:36, 20 January 2016 diff hist −159 Chromatin Immunoprecipitation →Immunoprecipitation: fixed numbering
• 16:2616:26, 20 January 2016 diff hist +83 N Category:Immunoprecipitation Created page with "These protocols are for both protein and chromatin immunoprecipitation experiments." current
• 16:2616:26, 20 January 2016 diff hist +410 N Farnham Lysis Buffer Wrote page for this buffer current
• 15:2715:27, 20 January 2016 diff hist +349 m Chromatin Immunoprecipitation Made section headers
• 15:2315:23, 20 January 2016 diff hist +78 Chromatin Immunoprecipitation →Buffers and Solutions Needed: made subsections
• 15:2215:22, 20 January 2016 diff hist +913 N Chromatin Immunoprecipitation Wrote initial section with buffers and link to new methods paper

17 January 2016

• 00:3700:37, 17 January 2016 diff hist +416 Bridges Lab Protocols:About Updated the about page

29 December 2015

• 20:5720:57, 29 December 2015 diff hist +1,569 N SHROB Genotyping wrote initial page

8 December 2015

• 17:5817:58, 8 December 2015 diff hist +52 First Strand cDNA Synthesis (AB Kit) Added AB Reaction protocol

25 September 2015

• 17:4217:42, 25 September 2015 diff hist +164 Extraction of DNA from TRIZOL preparations added categories, removed table of contents current

20 July 2015

• 21:1621:16, 20 July 2015 diff hist +8 m Quantification of miRNA by SYBR Green qPCR removed table of contents
• 21:0521:05, 20 July 2015 diff hist +609 Quantification of miRNA by SYBR Green qPCR Filled in details about reverse transcriptase
• 20:5620:56, 20 July 2015 diff hist +988 N Quantification of miRNA by SYBR Green qPCR copied protocol from Pfeffer paper

17 July 2015

• 13:4313:43, 17 July 2015 diff hist +745 N PCR Amplification with Platinum Taq High Fidelity copied from surveyor assay protocol current

14 July 2015

• 12:4912:49, 14 July 2015 diff hist +1,921 N Cloning CRISPR-Cas Plasmids copied over original page
• 12:4812:48, 14 July 2015 diff hist −333 Designing and Generating CRISPR-Cas Mutants removed stuff about double nickase current
• 12:4712:47, 14 July 2015 diff hist −1,726 Designing and Generating CRISPR-Cas Mutants Removed Cloning Protocol

13 July 2015

• 20:0220:02, 13 July 2015 diff hist +112 Surveyor Assay for Genomic DNA Mutations added categories current

24 June 2015

• 13:5613:56, 24 June 2015 diff hist 0 m Surveyor Assay for Genomic DNA Mutations changed volume of polymerase

26 May 2015

• 18:0118:01, 26 May 2015 diff hist +1,631 N Surveyor Assay for Genomic DNA Mutations wrote initial protocol

20 April 2015

• 15:3015:30, 20 April 2015 diff hist −1 m Oil red o stain for in vitro adipogenesis fixed isoproterenol format error current
• 15:2915:29, 20 April 2015 diff hist −48 m Oil red o stain for in vitro adipogenesis removed redundant title
• 15:2915:29, 20 April 2015 diff hist +50 m Oil red o stain for in vitro adipogenesis updated format

19 February 2015

• 14:4414:44, 19 February 2015 diff hist +4 m Paraffin Embedding of Tissue Samples No edit summary
• 14:4314:43, 19 February 2015 diff hist +753 Paraffin Embedding of Tissue Samples split table to allow for entering times

30 December 2014

• 18:5218:52, 30 December 2014 diff hist +1,491 N TaqMan miRNA Assay wrote first half current

29 December 2014

• 21:3521:35, 29 December 2014 diff hist +1,794 N Purification of miRNA and mRNA with TRIzol wrote initial page current

1 December 2014

• 21:4421:44, 1 December 2014 diff hist +486 N Tamoxifen Injections for Inducible Knockouts wrote initial page

11 November 2014

• 14:5714:57, 11 November 2014 diff hist +119 Primary Adipocyte Isolation added categories and RPM current