User contributions for Davebrid

Results for Davebrid talk block log uploads logs

A user with 938 edits. Account created on 2 August 2025.

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20:5720:57, 27 January 2016 diff hist +61 RIPA Buffer →RIPA Buffer (for 10mL lysis buffer): added link to orthovanadate protocol current
20:5720:57, 27 January 2016 diff hist +61 Preparing Cell Lysates →RIPA Buffer (for 10mL lysis buffer): added link to orthovanadate protocol current
20:5620:56, 27 January 2016 diff hist +831 N Preparation of Orthovanadate Stocks Wrote initial page
17:3517:35, 27 January 2016 diff hist +97 m Assessing isoproterenol-stimulated whole-body lipolysis in vivo Added categories

21:1021:10, 20 January 2016 diff hist +23 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA: specified tube for sonicaiton
18:3918:39, 20 January 2016 diff hist −2 Chromatin Immunoprecipitation →Crosslinking, Lysis and Shearing of DNA
18:3618:36, 20 January 2016 diff hist −159 Chromatin Immunoprecipitation →Immunoprecipitation: fixed numbering
16:2616:26, 20 January 2016 diff hist +83 N Category:Immunoprecipitation Created page with "These protocols are for both protein and chromatin immunoprecipitation experiments." current
16:2616:26, 20 January 2016 diff hist +410 N Farnham Lysis Buffer Wrote page for this buffer current
15:2715:27, 20 January 2016 diff hist +349 m Chromatin Immunoprecipitation Made section headers
15:2315:23, 20 January 2016 diff hist +78 Chromatin Immunoprecipitation →Buffers and Solutions Needed: made subsections
15:2215:22, 20 January 2016 diff hist +913 N Chromatin Immunoprecipitation Wrote initial section with buffers and link to new methods paper

00:3700:37, 17 January 2016 diff hist +416 Bridges Lab Protocols:About Updated the about page

20:5720:57, 29 December 2015 diff hist +1,569 N SHROB Genotyping wrote initial page

17:5817:58, 8 December 2015 diff hist +52 First Strand cDNA Synthesis (AB Kit) Added AB Reaction protocol

17:4217:42, 25 September 2015 diff hist +164 Extraction of DNA from TRIZOL preparations added categories, removed table of contents current

21:1621:16, 20 July 2015 diff hist +8 m Quantification of miRNA by SYBR Green qPCR removed table of contents
21:0521:05, 20 July 2015 diff hist +609 Quantification of miRNA by SYBR Green qPCR Filled in details about reverse transcriptase
20:5620:56, 20 July 2015 diff hist +988 N Quantification of miRNA by SYBR Green qPCR copied protocol from Pfeffer paper

13:4313:43, 17 July 2015 diff hist +745 N PCR Amplification with Platinum Taq High Fidelity copied from surveyor assay protocol current

12:4912:49, 14 July 2015 diff hist +1,921 N Cloning CRISPR-Cas Plasmids copied over original page
12:4812:48, 14 July 2015 diff hist −333 Designing and Generating CRISPR-Cas Mutants removed stuff about double nickase current
12:4712:47, 14 July 2015 diff hist −1,726 Designing and Generating CRISPR-Cas Mutants Removed Cloning Protocol

20:0220:02, 13 July 2015 diff hist +112 Surveyor Assay for Genomic DNA Mutations added categories current

13:5613:56, 24 June 2015 diff hist 0 m Surveyor Assay for Genomic DNA Mutations changed volume of polymerase

18:0118:01, 26 May 2015 diff hist +1,631 N Surveyor Assay for Genomic DNA Mutations wrote initial protocol

15:3015:30, 20 April 2015 diff hist −1 m Oil red o stain for in vitro adipogenesis fixed isoproterenol format error current
15:2915:29, 20 April 2015 diff hist −48 m Oil red o stain for in vitro adipogenesis removed redundant title
15:2915:29, 20 April 2015 diff hist +50 m Oil red o stain for in vitro adipogenesis updated format

14:4414:44, 19 February 2015 diff hist +4 m Paraffin Embedding of Tissue Samples No edit summary
14:4314:43, 19 February 2015 diff hist +753 Paraffin Embedding of Tissue Samples split table to allow for entering times

18:5218:52, 30 December 2014 diff hist +1,491 N TaqMan miRNA Assay wrote first half current

21:3521:35, 29 December 2014 diff hist +1,794 N Purification of miRNA and mRNA with TRIzol wrote initial page current

21:4421:44, 1 December 2014 diff hist +486 N Tamoxifen Injections for Inducible Knockouts wrote initial page

14:5714:57, 11 November 2014 diff hist +119 Primary Adipocyte Isolation added categories and RPM current
14:5114:51, 11 November 2014 diff hist +1,685 N Lipolysis Assay from Primary Adipocytes wrote initial page current
14:2514:25, 11 November 2014 diff hist +1,351 N Primary Adipocyte Isolation wrote initial page

14:1614:16, 29 October 2014 diff hist +12 Designing and Generating CRISPR-Cas Mutants updated with links to CRISPR design tool
13:5913:59, 29 October 2014 diff hist +219 Designing and Generating CRISPR-Cas Mutants updated with pX459

22:1222:12, 17 September 2014 diff hist +78 QPCR added booking site

14:5114:51, 31 July 2014 diff hist +125 Primer Design for qPCR updated specifying to start with the ncbi gene page

13:1213:12, 11 July 2014 diff hist +148 N Actinomycin D wrote initial page current
12:5512:55, 11 July 2014 diff hist +156 N Cycloheximide created page current

12:5012:50, 24 June 2014 diff hist +20 Glycogen Determination from Tissues updated product number for amyloglucosidase

12:3312:33, 29 April 2014 diff hist +1,961 N Triglyceride Assay from Tissue Culture Cells wrote protocol for cells
12:3012:30, 29 April 2014 diff hist +47 Triglyceride Assay from Cells and Tissues Undo revision 853 by Davebridges (talk)
12:3012:30, 29 April 2014 diff hist −47 Triglyceride Assay from Cells and Tissues Updated protocol for tissue culture

10:4310:43, 10 April 2014 diff hist 0 High Fat Diet Treatment updated caloric content of HFD

14:3814:38, 9 March 2014 diff hist +618 Designing and Generating CRISPR-Cas Mutants added notes about verifying clones

15:5315:53, 6 March 2014 diff hist +262 Designing and Generating CRISPR-Cas Mutants added note about naming of gDNA sequences

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