User contributions for Davebrid

6 March 2014

• 15:1415:14, 6 March 2014 diff hist +307 Designing and Generating CRISPR-Cas Mutants →Designing the Targeting Strategy: added note about avoiding spanning an intron

5 March 2014

• 18:3418:34, 5 March 2014 diff hist +646 N Sending Samples for DNA Sequencing Created page with "# Create an account or log in at http://128.169.4.12/ # Select Economy under Service Requested and enter the number of samples in the box above. # Select '''Enter Individual ..." current

1 March 2014

• 16:2216:22, 1 March 2014 diff hist +68 N Category:Cloning Created page with "This includes protocols for cloning and working with recombinant DNA" current
• 16:2216:22, 1 March 2014 diff hist +49 m Main Page →Cloning and Molecular Biology: added crispr cloning to index page
• 16:1916:19, 1 March 2014 diff hist +254 N Talk:Designing and Generating CRISPR-Cas Mutants added notes about diluting insert current
• 16:1716:17, 1 March 2014 diff hist +205 Designing and Generating CRISPR-Cas Mutants updated protocol after successful cloning

26 February 2014

• 21:0221:02, 26 February 2014 diff hist +127 Designing and Generating CRISPR-Cas Mutants added categories
• 21:0021:00, 26 February 2014 diff hist +59 Designing and Generating CRISPR-Cas Mutants updated annealing conditions
• 20:2820:28, 26 February 2014 diff hist +840 Designing and Generating CRISPR-Cas Mutants Added cloning information
• 18:0118:01, 26 February 2014 diff hist +8 Designing and Generating CRISPR-Cas Mutants updated image schematic
• 17:5417:54, 26 February 2014 diff hist +95 N File:PX335.png Image of pX335 multiple cloning site, taken from the Addgene site http://www.addgene.org/42335/ current
• 17:5317:53, 26 February 2014 diff hist +1,856 N Designing and Generating CRISPR-Cas Mutants added initial page

14 February 2014

• 17:2017:20, 14 February 2014 diff hist +37 Measuring Grip Strength added blanking step current
• 17:2017:20, 14 February 2014 diff hist +1,135 N Measuring Grip Strength wrote grip protocol

12 February 2014

• 16:0716:07, 12 February 2014 diff hist +920 N Making LB Agar Plates wrote page for making plates
• 15:4415:44, 12 February 2014 diff hist −115 Transformation of Bacteria updated transformation protocol current

10 February 2014

• 16:5816:58, 10 February 2014 diff hist +4 Splitting Cells added temperature

28 January 2014

• 21:2121:21, 28 January 2014 diff hist 0 Amitryptyline Treatment of Mice No edit summary current
• 21:1921:19, 28 January 2014 diff hist +2 m Amitryptyline Treatment of Mice fixed two typos

23 January 2014

• 16:0416:04, 23 January 2014 diff hist +668 N Amitryptyline Treatment of Mice wrote protocol for amitriptyline in water

17 January 2014

• 16:3916:39, 17 January 2014 diff hist 0 m Insulin Tolerance Test fixed glucose typo
• 15:2915:29, 17 January 2014 diff hist +61 Insulin Tolerance Test added ordering details for humulin

5 December 2013

• 14:2214:22, 5 December 2013 diff hist +491 Using Github and Git for Code updated with instructions about pulling in from remote repositories. current

2 December 2013

• 14:5714:57, 2 December 2013 diff hist +1,583 N Quantifying Relative Expression from qRT-PCR Wrote initial page current

31 October 2013

• 13:5213:52, 31 October 2013 diff hist +14 Primer Design for qPCR added direction to primer name
• 13:3813:38, 31 October 2013 diff hist +2,251 N QPCR Wrote page for qPCR

15 October 2013

• 13:1013:10, 15 October 2013 diff hist +26 Parsing XSQ Files added filenames to command current

14 October 2013

• 21:3221:32, 14 October 2013 diff hist +1,095 N Primer Design for qPCR wrote protocol
• 21:1521:15, 14 October 2013 diff hist +644 N Parsing XSQ Files copied out instructions.

7 October 2013

• 17:1717:17, 7 October 2013 diff hist +216 N Creating New R Packages wrote new page current

26 September 2013

• 19:5919:59, 26 September 2013 diff hist +482 Ob/ob Genotyping added nots on how to make primer mixes and added band sizes current

23 September 2013

• 14:1714:17, 23 September 2013 diff hist +13 Differentiation of 3T3-L1 Cells updated MIX stock solution instructions

18 September 2013

• 15:0715:07, 18 September 2013 diff hist +82 m Rapamycin Stock Solution added categories
• 15:0615:06, 18 September 2013 diff hist +285 N Rapamycin Stock Solution wrote initial page

12 September 2013

• 15:5515:55, 12 September 2013 diff hist +436 N Culturing MIN6 Cells wrote initial page current
• 15:0415:04, 12 September 2013 diff hist +485 N Measuring Autophagy in Tissue Culture Cells added notes about bafilomycin treatment current

27 August 2013

• 13:5213:52, 27 August 2013 diff hist +135 High Fat Diet Treatment added note about ordering C57BL/6J mice

16 July 2013

• 00:2400:24, 16 July 2013 diff hist −1 Bridges Lab Protocols:About No edit summary

15 July 2013

• 16:1116:11, 15 July 2013 diff hist +338 Preparation of Protein Lysates from Mouse Tissues updated protocol with new weights

9 July 2013

• 14:3714:37, 9 July 2013 diff hist +120 m Cre PCR added categories
• 14:3614:36, 9 July 2013 diff hist −2 m Cre PCR No edit summary
• 14:3514:35, 9 July 2013 diff hist +96 N Cre PCR copied in PCR program
• 14:3414:34, 9 July 2013 diff hist +7 Genotyping Program added link to Cre PCR program

5 July 2013

• 14:5514:55, 5 July 2013 diff hist +495 N TC10b Genotyping wrote initial protocol current

19 June 2013

• 15:5315:53, 19 June 2013 diff hist +131 N Bridges Lab Protocols:Copyrights added link to CC0 for copyrights page current
• 15:4215:42, 19 June 2013 diff hist +546 Main Page Wrote greeting and reformatted main page

12 April 2013

• 15:0715:07, 12 April 2013 diff hist +90 Western Blotting added links to buffers
• 15:0515:05, 12 April 2013 diff hist +366 N SDS-PAGE Running Buffer copied recipe from saltiel lab buffers current
• 14:5714:57, 12 April 2013 diff hist +357 N Transfer Buffer copied recipe from saltiel lab buffers
• 14:5214:52, 12 April 2013 diff hist +344 N TBST copied recipe from saltiel lab buffers current