User contributions for Davebrid

22 February 2013

• 16:5716:57, 22 February 2013 diff hist +34 m Lipofectamine Transfection of C2C12 Cells changed confluence note current
• 16:5616:56, 22 February 2013 diff hist +1,325 N Lipofectamine Transfection of C2C12 Cells Wrote protocol from ethan's trials
• 16:4516:45, 22 February 2013 diff hist +446 N Talk:Fugene Transfection of C2C12 Cells ethan provided his protocol current

18 February 2013

• 20:4220:42, 18 February 2013 diff hist +28 Glucose Uptake Assay specified insulin concentration

21 January 2013

• 15:2015:20, 21 January 2013 diff hist +96 Genotyping Program added MTM1 program
• 15:1715:17, 21 January 2013 diff hist +120 m MTM1 Genotyping Added categories current
• 15:1615:16, 21 January 2013 diff hist +42 MTM1 Genotyping added sample pcr result
• 15:1515:15, 21 January 2013 diff hist +158 N File:MTM1 Genotyping Result.jpg Expected results of Genotyping program, from http://jaxmice.jax.org/protocolsdb/f?p=116:2:3785778730065538::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:8261,018153 current
• 15:1315:13, 21 January 2013 diff hist +501 N MTM1 Genotyping wrote protocol for MTM1 genotyping

11 January 2013

• 14:5814:58, 11 January 2013 diff hist +66 Maintenance of Fly Stocks added food cost current

30 December 2012

• 19:1119:11, 30 December 2012 diff hist +142 m TSC-floxed Genotyping added categories current
• 19:1019:10, 30 December 2012 diff hist +891 N TSC-floxed Genotyping Added primers and reference for TSC1 primers

14 December 2012

• 18:3818:38, 14 December 2012 diff hist +66 N Talk:Triglyceride Assay from Cells and Tissues added alternative protocol from ron kahn current

4 December 2012

• 21:0421:04, 4 December 2012 diff hist 0 5' RACE →PRC of dC-Tailed cDNA current
• 21:0421:04, 4 December 2012 diff hist +822 5' RACE Added PCR reaction
• 14:4914:49, 4 December 2012 diff hist +1,048 5' RACE Added purification and tailing protocols
• 14:3614:36, 4 December 2012 diff hist +1,599 N 5' RACE Rewrote protcol for first strand synthesis

30 November 2012

• 14:5114:51, 30 November 2012 diff hist +1,499 N Measuring Lifespan in Drosophila wrote initial protocol based on Broughton et al current
• 14:2314:23, 30 November 2012 diff hist +11 m Talk:Measuring Lifespan in Drosophila updated link to PLOS paper current

29 November 2012

• 18:1018:10, 29 November 2012 diff hist +920 Talk:Measuring Lifespan in Drosophila added ason's papers

25 November 2012

• 17:4717:47, 25 November 2012 diff hist +768 N Talk:Measuring Lifespan in Drosophila added papers suggested by matt piper

21 November 2012

• 19:2919:29, 21 November 2012 diff hist +112 Maintenance of Fly Stocks added note about expanding colonies

20 November 2012

• 15:0815:08, 20 November 2012 diff hist +1 m High Fat Diet Treatment error in sucrose amount
• 14:5314:53, 20 November 2012 diff hist +166 m High Fat Diet Treatment added header and categories
• 14:5114:51, 20 November 2012 diff hist +1,375 N High Fat Diet Treatment filled in details about mouse diets

16 November 2012

• 20:5520:55, 16 November 2012 diff hist +37 N Category:Drosophila Created page with 'Protocols for Drosophila experiments.' current
• 20:5520:55, 16 November 2012 diff hist +44 Nm Talk:Maintenance of Fly Stocks Created page with 'Taught by Ason Chiang from the Yamashita Lab' current
• 20:5420:54, 16 November 2012 diff hist +1,087 N Maintenance of Fly Stocks wrote initial protocol

5 November 2012

• 16:3116:31, 5 November 2012 diff hist +35 Rapamycin Injections updated vehicle information
• 16:2316:23, 5 November 2012 diff hist +77 Talk:Rapamycin Injections added kunliang paper current
• 16:0916:09, 5 November 2012 diff hist +187 N Talk:Rapamycin Injections Created page with '==Other Dosing Schedules== ===Vehicles=== * 0.25% PEG 400/0.25% Tween-80 in http://dx.doi.org/10.1186/1479-5876-8-14 * 4% PEG 200/4% Tween-80 in http://dx.doi.org/10.1073/pnas.1...'

23 October 2012

• 15:2215:22, 23 October 2012 diff hist +19 Genotyping Program added note about band size in cap ko

10 October 2012

• 15:0915:09, 10 October 2012 diff hist 0 m Ob/ob Genotyping changed program name

9 October 2012

• 16:4416:44, 9 October 2012 diff hist +151 Genotyping Program added ob/ob genotyping
• 16:4216:42, 9 October 2012 diff hist +53 Ob/ob Genotyping added other gene specific primer
• 16:1516:15, 9 October 2012 diff hist +63 N File:Ob ob.jpg summary of bands from ob/ob genotyping. Taken from Ellet et al current
• 16:1416:14, 9 October 2012 diff hist +30 Ob/ob Genotyping No edit summary
• 16:0316:03, 9 October 2012 diff hist +119 Ob/ob Genotyping added categories
• 16:0216:02, 9 October 2012 diff hist +401 N Ob/ob Genotyping wrote genotyping protocol from Ellet et al

3 October 2012

• 21:0121:01, 3 October 2012 diff hist +135 Using the MBNI Cluster added command to specify RAM minimum for a submitted job current

2 October 2012

• 16:5116:51, 2 October 2012 diff hist +335 N Using the MBNI Cluster added qstat/qhost/qsub/qdel commands

20 September 2012

• 19:5919:59, 20 September 2012 diff hist 0 Purification of GST-HA-S6K updated dna amount in materials (was ok in protocol) current

18 September 2012

• 18:5318:53, 18 September 2012 diff hist +1 Triglyceride Assay from Cells and Tissues changed sigma catalog number

5 September 2012

• 15:5015:50, 5 September 2012 diff hist +46 Isolation of Mouse Embryonic Fibroblasts (MEFs) updated with more details current

4 September 2012

• 16:3216:32, 4 September 2012 diff hist +695 N Isolation of Mouse Embryonic Fibroblasts (MEFs) copied in protocol from sergey's paper

3 September 2012

• 17:3517:35, 3 September 2012 diff hist +469 Rapamycin Injections added rapamycin treatment from Lamming et al

22 August 2012

• 18:2618:26, 22 August 2012 diff hist −46 m Purification of GST-HA-S6K No edit summary
• 18:1818:18, 22 August 2012 diff hist +197 Purification of GST-HA-S6K updated lipofectamine conditions

15 August 2012

• 18:1918:19, 15 August 2012 diff hist −273 Culturing and Differentiating C2C12 Cells added differentiation protocol
• 18:1118:11, 15 August 2012 diff hist +1,012 N Talk:Culturing and Differentiating C2C12 Cells added atcc notes to history current