User contributions for Davebrid

9 October 2012

• 16:1516:15, 9 October 2012 diff hist +63 N File:Ob ob.jpg summary of bands from ob/ob genotyping. Taken from Ellet et al current
• 16:1416:14, 9 October 2012 diff hist +30 Ob/ob Genotyping No edit summary
• 16:0316:03, 9 October 2012 diff hist +119 Ob/ob Genotyping added categories
• 16:0216:02, 9 October 2012 diff hist +401 N Ob/ob Genotyping wrote genotyping protocol from Ellet et al

3 October 2012

• 21:0121:01, 3 October 2012 diff hist +135 Using the MBNI Cluster added command to specify RAM minimum for a submitted job current

2 October 2012

• 16:5116:51, 2 October 2012 diff hist +335 N Using the MBNI Cluster added qstat/qhost/qsub/qdel commands

20 September 2012

• 19:5919:59, 20 September 2012 diff hist 0 Purification of GST-HA-S6K updated dna amount in materials (was ok in protocol) current

18 September 2012

• 18:5318:53, 18 September 2012 diff hist +1 Triglyceride Assay from Cells and Tissues changed sigma catalog number

5 September 2012

• 15:5015:50, 5 September 2012 diff hist +46 Isolation of Mouse Embryonic Fibroblasts (MEFs) updated with more details current

4 September 2012

• 16:3216:32, 4 September 2012 diff hist +695 N Isolation of Mouse Embryonic Fibroblasts (MEFs) copied in protocol from sergey's paper

3 September 2012

• 17:3517:35, 3 September 2012 diff hist +469 Rapamycin Injections added rapamycin treatment from Lamming et al

22 August 2012

• 18:2618:26, 22 August 2012 diff hist −46 m Purification of GST-HA-S6K No edit summary
• 18:1818:18, 22 August 2012 diff hist +197 Purification of GST-HA-S6K updated lipofectamine conditions

15 August 2012

• 18:1918:19, 15 August 2012 diff hist −273 Culturing and Differentiating C2C12 Cells added differentiation protocol
• 18:1118:11, 15 August 2012 diff hist +1,012 N Talk:Culturing and Differentiating C2C12 Cells added atcc notes to history current

8 August 2012

• 14:1114:11, 8 August 2012 diff hist +84 m Culturing and Differentiating C2C12 Cells added categories
• 14:0914:09, 8 August 2012 diff hist +1,150 N Culturing and Differentiating C2C12 Cells wrote initial protocol

6 August 2012

• 15:1415:14, 6 August 2012 diff hist +120 Vinexin Genotyping Added categories current
• 15:1315:13, 6 August 2012 diff hist +247 Vinexin Genotyping Revised adding sections for Primers and PCR Program
• 14:4214:42, 6 August 2012 diff hist +1,323 N Vinexin Genotyping pasted in protocol from Vinexin genotyping paper
• 14:4014:40, 6 August 2012 diff hist +52 Genotyping Program Added vinexin

30 July 2012

• 16:0616:06, 30 July 2012 diff hist −7 m Glucose Incorporation into Lipid and Glycogen fixed typos in links current
• 16:0516:05, 30 July 2012 diff hist +70 Glucose Incorporation into Lipid and Glycogen added reference

24 July 2012

• 17:5517:55, 24 July 2012 diff hist +10 m Purification of GST Fusion Proteins by GSTrap removed table of contents current
• 17:5417:54, 24 July 2012 diff hist +80 Induction Conditions added Rab5 current
• 17:5317:53, 24 July 2012 diff hist +34 N Category:Protein Purification Created page with 'Protocols for protein purification' current
• 17:5317:53, 24 July 2012 diff hist +994 Purification of GST Fusion Proteins by GSTrap →FPLC Run: Added details about HPLC run
• 17:3917:39, 24 July 2012 diff hist +1,714 N Purification of GST Fusion Proteins by GSTrap Added initial protocol, need to add details about HPLC Run

23 July 2012

• 18:5618:56, 23 July 2012 diff hist −44 Transformation of Bacteria removed confusing buffer addition

21 July 2012

• 11:3211:32, 21 July 2012 diff hist +49 N Category:Bioinformatics wrote summary for page current
• 11:3111:31, 21 July 2012 diff hist +96 m Installing Software to a UNIX Server added categories current
• 11:3011:30, 21 July 2012 diff hist −2 m Installing Software to a UNIX Server corrected a link
• 11:3011:30, 21 July 2012 diff hist +899 N Installing Software to a UNIX Server wrote intial page

29 June 2012

• 19:2119:21, 29 June 2012 diff hist +65 Back-Crossing of Mouse Strains specified to keep backcrossing current
• 19:0319:03, 29 June 2012 diff hist +107 Ordering Mice added ULAM mouse ordering form current

26 June 2012

• 18:1018:10, 26 June 2012 diff hist +384 Statistical Analysis added section about accounting for other effects within the Interaction section current
• 18:0418:04, 26 June 2012 diff hist +411 m Statistical Analysis re-worded several sentences
• 17:3317:33, 26 June 2012 diff hist 0 m Statistical Analysis typo for comparison
• 17:3217:32, 26 June 2012 diff hist +43 Statistical Analysis added categories
• 17:3017:30, 26 June 2012 diff hist +5,479 N Statistical Analysis wrote most of initial stats page

12 June 2012

• 17:2517:25, 12 June 2012 diff hist +416 N Phred Quality Scores copied from wikipedia current

11 June 2012

• 15:5515:55, 11 June 2012 diff hist +76 N Category:Genotyping Created page with 'This page lists protocols for genotyping of various animal and cell strains.' current
• 15:5515:55, 11 June 2012 diff hist +85 N Ordering Mice wrote use category
• 15:5415:54, 11 June 2012 diff hist +93 Back-Crossing of Mouse Strains added categories and link to ordering mice
• 15:4415:44, 11 June 2012 diff hist +1,502 N Back-Crossing of Mouse Strains wrote page for back-cross protocol

1 June 2012

• 16:4616:46, 1 June 2012 diff hist +18 m Yeast Selective Media and Agar updated categories current
• 16:4616:46, 1 June 2012 diff hist +18 m YPD Media and Agar changed categories current
• 16:4516:45, 1 June 2012 diff hist +105 m First Strand cDNA Synthesis (AB Kit) added categories
• 16:2716:27, 1 June 2012 diff hist +795 N First Strand cDNA Synthesis (AB Kit) Wrote protocol for AB Kit

31 May 2012

• 15:5515:55, 31 May 2012 diff hist +38 N Category:QPCR added description current