User contributions for Davebrid

31 May 2012

• 15:5515:55, 31 May 2012 diff hist 0 Preparation of RNA Samples from Mouse Tissues changed volume to remove of upper phase (typo from original protocol)
• 15:5415:54, 31 May 2012 diff hist +4,769 N Talk:Preparation of RNA Samples from Mouse Tissues added original protocol to talk page current

28 May 2012

• 15:0715:07, 28 May 2012 diff hist +6 m Glycogen Determination from Tissues changed category
• 15:0615:06, 28 May 2012 diff hist +6 m Dexamethasone Treatment of Mice changed category
• 15:0615:06, 28 May 2012 diff hist −19 m Main Page changed link to category page
• 14:4614:46, 28 May 2012 diff hist +112 Main Page added categories
• 14:4414:44, 28 May 2012 diff hist +50 m Genotyping Program added categories
• 14:4314:43, 28 May 2012 diff hist +101 m Preparation of Tail Samples (for Genotyping) added categories
• 14:4314:43, 28 May 2012 diff hist +51 m PCR Analysis of Tail DNA added categories
• 14:4214:42, 28 May 2012 diff hist +5 m Preparation of RNA Samples from Mouse Tissues changed category
• 14:4214:42, 28 May 2012 diff hist +5 m Preparation of Protein Lysates from Mouse Tissues changed category
• 14:4114:41, 28 May 2012 diff hist +32 N Category:Mouse Work filled in category page for mouse work current
• 14:1514:15, 28 May 2012 diff hist +567 Triglyceride Assay from Cells and Tissues updated volumes and added a new section describing tissue specific volumes to use
• 13:5913:59, 28 May 2012 diff hist +33 m Amylose pull-down of glycogen-bound proteins added formatting current

21 May 2012

• 14:4014:40, 21 May 2012 diff hist +145 Glycogen Determination from Tissues added sweave template

18 May 2012

• 18:2118:21, 18 May 2012 diff hist +6 m Glycogen Determination from Tissues bolded vial type
• 13:0213:02, 18 May 2012 diff hist −2 m Glycogen Synthase Assay updated category current
• 13:0213:02, 18 May 2012 diff hist +75 N Category:Cell Culture Created page with 'This page contains protocols related to cell an tissue culture experiments.' current

13 April 2012

• 16:0416:04, 13 April 2012 diff hist +170 Triglyceride Assay from Cells and Tissues updated with note about using less tissue
• 16:0316:03, 13 April 2012 diff hist +995 Triglyceride Assay from Cells and Tissues added details about using the sigma kit

4 April 2012

• 17:1217:12, 4 April 2012 diff hist +30 m Immunoprecipitation updated formatting

5 March 2012

• 21:0421:04, 5 March 2012 diff hist +8 m Using Github and Git for Code made references into a list
• 21:0321:03, 5 March 2012 diff hist +1,806 N Using Github and Git for Code wori

27 February 2012

• 17:2617:26, 27 February 2012 diff hist +281 N CAP Genotyping wrote page for CAP genotyping current
• 17:2317:23, 27 February 2012 diff hist +155 Genotyping Program Updated table, added links to specific genotyping protocols
• 15:0615:06, 27 February 2012 diff hist +45 m Preparation of Tail Samples (for Genotyping) added markup

21 February 2012

• 16:3916:39, 21 February 2012 diff hist +78 m Verifying Kan Deletion Mutants from Genomic DNA Added categories current
• 16:3916:39, 21 February 2012 diff hist +16 m Verifying Kan Deletion Mutants from Genomic DNA changed PCR reaction to a list
• 16:3816:38, 21 February 2012 diff hist +1,427 N Verifying Kan Deletion Mutants from Genomic DNA copied protocol from emily kauffman
• 16:3216:32, 21 February 2012 diff hist +1,305 N Rapid Isolation of Genomic DNA from Yeast copied protocol from emily kauffman current

8 February 2012

• 22:3722:37, 8 February 2012 diff hist +173 Designing siRNA for Knockdown added instructions for reordering a siRNA current

17 January 2012

• 22:0322:03, 17 January 2012 diff hist +3 Cesium Chloride Preparation of DNA updated cesium concentration current

13 January 2012

• 16:4216:42, 13 January 2012 diff hist +40 m Glycogen Determination from Tissues described preparation and storage of amyloglucosidase

11 January 2012

• 17:0017:00, 11 January 2012 diff hist +47 Preparation of RNA Samples from Mouse Tissues added ethanol to materials and prepared TRIzol tubes first
• 16:5116:51, 11 January 2012 diff hist +939 Preparation of RNA Samples from Mouse Tissues updated for purelink kits.

9 January 2012

• 18:4618:46, 9 January 2012 diff hist +1 Designing siRNA for Knockdown updated quote

3 January 2012

• 17:5017:50, 3 January 2012 diff hist +3 m Ordering Barcodes for Mice typo in binbins row current
• 17:4917:49, 3 January 2012 diff hist +681 N Ordering Barcodes for Mice initial page

11 November 2011

• 21:1621:16, 11 November 2011 diff hist −1 m Glucose Incorporation into Lipid and Glycogen changed template link
• 21:1521:15, 11 November 2011 diff hist +390 Glucose Incorporation into Lipid and Glycogen added some extra notes and a link to the template

8 November 2011

• 16:0816:08, 8 November 2011 diff hist +207 RNA-seq Pipeline for Known Transcripts added section for aligning reads with bowtie current
• 16:0216:02, 8 November 2011 diff hist +73 RNA-seq Pipeline for Known Transcripts added seqanswers link

3 November 2011

• 13:4413:44, 3 November 2011 diff hist +323 Glucose Uptake Assay added templates

2 November 2011

• 15:1615:16, 2 November 2011 diff hist +415 N Talk:Dexamethasone Treatment of Mice added note about protocol source current
• 15:1515:15, 2 November 2011 diff hist +1,052 N Dexamethasone Treatment of Mice wrote initial page

21 October 2011

• 13:3113:31, 21 October 2011 diff hist −4 m RNA-seq Pipeline for Known Transcripts removed linebreak
• 13:3013:30, 21 October 2011 diff hist +959 RNA-seq Pipeline for Known Transcripts added instructions for barcode splitting

3 October 2011

• 20:2220:22, 3 October 2011 diff hist +1,165 N Adding New Personnel to Animal Protocol Copied over from Shannon's Instructions current
• 20:1320:13, 3 October 2011 diff hist +159 m RNA-seq Pipeline for Known Transcripts added link to FastQC report analysis
• 20:1220:12, 3 October 2011 diff hist +244 RNA-seq Pipeline for Known Transcripts added information about using FastQC