User contributions for Davebrid

Results for Davebrid talk block log uploads logs

A user with 938 edits. Account created on 2 August 2025.

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18:2118:21, 18 May 2012 diff hist +6 m Glycogen Determination from Tissues bolded vial type
13:0213:02, 18 May 2012 diff hist −2 m Glycogen Synthase Assay updated category current
13:0213:02, 18 May 2012 diff hist +75 N Category:Cell Culture Created page with 'This page contains protocols related to cell an tissue culture experiments.' current

16:0416:04, 13 April 2012 diff hist +170 Triglyceride Assay from Cells and Tissues updated with note about using less tissue
16:0316:03, 13 April 2012 diff hist +995 Triglyceride Assay from Cells and Tissues added details about using the sigma kit

17:1217:12, 4 April 2012 diff hist +30 m Immunoprecipitation updated formatting

21:0421:04, 5 March 2012 diff hist +8 m Using Github and Git for Code made references into a list
21:0321:03, 5 March 2012 diff hist +1,806 N Using Github and Git for Code wori

17:2617:26, 27 February 2012 diff hist +281 N CAP Genotyping wrote page for CAP genotyping current
17:2317:23, 27 February 2012 diff hist +155 Genotyping Program Updated table, added links to specific genotyping protocols
15:0615:06, 27 February 2012 diff hist +45 m Preparation of Tail Samples (for Genotyping) added markup

16:3916:39, 21 February 2012 diff hist +78 m Verifying Kan Deletion Mutants from Genomic DNA Added categories current
16:3916:39, 21 February 2012 diff hist +16 m Verifying Kan Deletion Mutants from Genomic DNA changed PCR reaction to a list
16:3816:38, 21 February 2012 diff hist +1,427 N Verifying Kan Deletion Mutants from Genomic DNA copied protocol from emily kauffman
16:3216:32, 21 February 2012 diff hist +1,305 N Rapid Isolation of Genomic DNA from Yeast copied protocol from emily kauffman current

22:3722:37, 8 February 2012 diff hist +173 Designing siRNA for Knockdown added instructions for reordering a siRNA current

22:0322:03, 17 January 2012 diff hist +3 Cesium Chloride Preparation of DNA updated cesium concentration current

16:4216:42, 13 January 2012 diff hist +40 m Glycogen Determination from Tissues described preparation and storage of amyloglucosidase

17:0017:00, 11 January 2012 diff hist +47 Preparation of RNA Samples from Mouse Tissues added ethanol to materials and prepared TRIzol tubes first
16:5116:51, 11 January 2012 diff hist +939 Preparation of RNA Samples from Mouse Tissues updated for purelink kits.

18:4618:46, 9 January 2012 diff hist +1 Designing siRNA for Knockdown updated quote

17:5017:50, 3 January 2012 diff hist +3 m Ordering Barcodes for Mice typo in binbins row current
17:4917:49, 3 January 2012 diff hist +681 N Ordering Barcodes for Mice initial page

21:1621:16, 11 November 2011 diff hist −1 m Glucose Incorporation into Lipid and Glycogen changed template link
21:1521:15, 11 November 2011 diff hist +390 Glucose Incorporation into Lipid and Glycogen added some extra notes and a link to the template

16:0816:08, 8 November 2011 diff hist +207 RNA-seq Pipeline for Known Transcripts added section for aligning reads with bowtie current
16:0216:02, 8 November 2011 diff hist +73 RNA-seq Pipeline for Known Transcripts added seqanswers link

13:4413:44, 3 November 2011 diff hist +323 Glucose Uptake Assay added templates

15:1615:16, 2 November 2011 diff hist +415 N Talk:Dexamethasone Treatment of Mice added note about protocol source current
15:1515:15, 2 November 2011 diff hist +1,052 N Dexamethasone Treatment of Mice wrote initial page

13:3113:31, 21 October 2011 diff hist −4 m RNA-seq Pipeline for Known Transcripts removed linebreak
13:3013:30, 21 October 2011 diff hist +959 RNA-seq Pipeline for Known Transcripts added instructions for barcode splitting

20:2220:22, 3 October 2011 diff hist +1,165 N Adding New Personnel to Animal Protocol Copied over from Shannon's Instructions current
20:1320:13, 3 October 2011 diff hist +159 m RNA-seq Pipeline for Known Transcripts added link to FastQC report analysis
20:1220:12, 3 October 2011 diff hist +244 RNA-seq Pipeline for Known Transcripts added information about using FastQC

16:4416:44, 19 September 2011 diff hist 0 m Glycogen Phosphorylase Assay typo in categories current
16:4216:42, 19 September 2011 diff hist +1,454 N Glycogen Phosphorylase Assay copied protocol from alan cheng

14:0014:00, 14 September 2011 diff hist +120 RNA-seq Pipeline for Known Transcripts added categories
13:3713:37, 14 September 2011 diff hist +236 RNA-seq Pipeline for Known Transcripts updated formatting
13:1513:15, 14 September 2011 diff hist +3,169 N RNA-seq Pipeline for Known Transcripts Pasted in Instructions from Richard McEachin

18:3918:39, 29 August 2011 diff hist +19 m Designing siRNA for Knockdown updated quote number
13:2213:22, 29 August 2011 diff hist +13 m Fugene Transfection of 293T/COS Cells added catalog number current
13:2113:21, 29 August 2011 diff hist +507 Fugene Transfection of 293T/COS Cells updated protocol

15:1715:17, 24 August 2011 diff hist −153 Immunofluoresence updated protocol

14:2814:28, 11 August 2011 diff hist +339 N Preparation of Tail Samples (for Genotyping) Created page with 'PBND Solution: Tail Lysis Buffer -50mM KCl -10mM Tris HCl (pH~8.3) -0.1 mg/mL MgCl2, 6H20 -0.1 mg/mL Gelatin -0.45% (NP-40) Jgepal -0.45% Tween 20 Protocol 1.) Combine 200uL o...'

18:4518:45, 10 August 2011 diff hist +14 Acetate Incorporation into Lipid rewrote procedure current

20:4320:43, 2 August 2011 diff hist +2,524 N Antisense Oligo Knockdown in Mice wrote initial page current

15:0315:03, 24 June 2011 diff hist +7 Glycogen Determination from Tissues No edit summary
15:0215:02, 24 June 2011 diff hist +379 Glycogen Determination from Tissues updated details about assay

18:0218:02, 23 June 2011 diff hist +28 Rapamycin Injections added dosage volume

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