User contributions for Davebrid

19 September 2011

• 16:4416:44, 19 September 2011 diff hist 0 m Glycogen Phosphorylase Assay typo in categories current
• 16:4216:42, 19 September 2011 diff hist +1,454 N Glycogen Phosphorylase Assay copied protocol from alan cheng

14 September 2011

• 14:0014:00, 14 September 2011 diff hist +120 RNA-seq Pipeline for Known Transcripts added categories
• 13:3713:37, 14 September 2011 diff hist +236 RNA-seq Pipeline for Known Transcripts updated formatting
• 13:1513:15, 14 September 2011 diff hist +3,169 N RNA-seq Pipeline for Known Transcripts Pasted in Instructions from Richard McEachin

29 August 2011

• 18:3918:39, 29 August 2011 diff hist +19 m Designing siRNA for Knockdown updated quote number
• 13:2213:22, 29 August 2011 diff hist +13 m Fugene Transfection of 293T/COS Cells added catalog number current
• 13:2113:21, 29 August 2011 diff hist +507 Fugene Transfection of 293T/COS Cells updated protocol

24 August 2011

• 15:1715:17, 24 August 2011 diff hist −153 Immunofluoresence updated protocol

11 August 2011

• 14:2814:28, 11 August 2011 diff hist +339 N Preparation of Tail Samples (for Genotyping) Created page with 'PBND Solution: Tail Lysis Buffer -50mM KCl -10mM Tris HCl (pH~8.3) -0.1 mg/mL MgCl2, 6H20 -0.1 mg/mL Gelatin -0.45% (NP-40) Jgepal -0.45% Tween 20 Protocol 1.) Combine 200uL o...'

10 August 2011

• 18:4518:45, 10 August 2011 diff hist +14 Acetate Incorporation into Lipid rewrote procedure current

2 August 2011

• 20:4320:43, 2 August 2011 diff hist +2,524 N Antisense Oligo Knockdown in Mice wrote initial page current

24 June 2011

• 15:0315:03, 24 June 2011 diff hist +7 Glycogen Determination from Tissues No edit summary
• 15:0215:02, 24 June 2011 diff hist +379 Glycogen Determination from Tissues updated details about assay

23 June 2011

• 18:0218:02, 23 June 2011 diff hist +28 Rapamycin Injections added dosage volume

18 May 2011

• 20:4520:45, 18 May 2011 diff hist +1,019 N Measurement of Glycolysis and Respiration Using Seahorse wrote initial page current

13 May 2011

• 15:0015:00, 13 May 2011 diff hist +220 Insulin Tolerance Test updated with new starvation and added additional information

20 April 2011

• 15:3115:31, 20 April 2011 diff hist +846 N Analysing Sequencing Results with SeqMan wrote initial page current

14 April 2011

• 14:4014:40, 14 April 2011 diff hist +1 Surface Plasmon Resonance - Protein Lipid Interactions updated protocol to make 300 uL of lipid to make extruding easier current

29 March 2011

• 00:4200:42, 29 March 2011 diff hist +107 Real Time PCR From Cell Culture added reference for calculating amounts from Ct current

16 March 2011

• 16:5316:53, 16 March 2011 diff hist +318 Colocalization updated protocol current

9 March 2011

• 19:5519:55, 9 March 2011 diff hist +720 N Lipid Transfection Created page with '==Materials== * Shuttle Kit from Echelon [http://www.echelon-inc.com/commerce/ccc1021-shuttle-pip-kits.htm] * I used C8 lipids and dissolved to 1 mM in PBS and frozen in small al...' current

1 March 2011

• 21:3321:33, 1 March 2011 diff hist −2 Acetate Incorporation into Lipid increased from 0.1 to 10 uCi per mL.

9 February 2011

• 19:4719:47, 9 February 2011 diff hist +177 Affinity Purification of Proteins - FPLC reworded some bits of the protocol current

2 February 2011

• 19:0419:04, 2 February 2011 diff hist +31 m Immunofluoresence added categories
• 19:0319:03, 2 February 2011 diff hist +57 N Category:Yeast Created page with 'Protocols using yeast, typically cell biology experiments' current

31 January 2011

• 20:5520:55, 31 January 2011 diff hist +73 Protein-Lipid Overlay Assay added categories current
• 20:5420:54, 31 January 2011 diff hist +1,147 N Protein-Lipid Overlay Assay copied protocol

12 January 2011

• 19:4619:46, 12 January 2011 diff hist +94 m Glucose Uptake Assay added categories

13 December 2010

• 19:4719:47, 13 December 2010 diff hist 0 Preparing an Adenoviral shRNA Clone →Choosing a shRNA Sequence: updated XbaI compatible overhang current

10 December 2010

• 21:4921:49, 10 December 2010 diff hist +33 Real Time PCR From Cell Culture altered primer concentration and volumes for 384 well plate
• 20:2320:23, 10 December 2010 diff hist +82 m Glucose Tolerance Test added categories

6 December 2010

• 17:1517:15, 6 December 2010 diff hist +53 Real Time PCR From Cell Culture added link to preparation of rna from tissue
• 15:0715:07, 6 December 2010 diff hist −70 m Genotyping Program No edit summary
• 15:0615:06, 6 December 2010 diff hist +497 N Genotyping Program Created page with '{| border="1" !| Allele || Program|| Fragment Size(s) || Notes |- |TSC-floxed || Inoki PCR || || Two bands if heterozygote |- |Cre || Inoki PCR || || Only present if Transgene...'
• 14:5014:50, 6 December 2010 diff hist +165 GoTaq PCR Genotyping updated protocol and added categories current

2 December 2010

• 22:0022:00, 2 December 2010 diff hist +2,189 N Purification of MBP Fusion Proteins wrote initial page current

22 November 2010

• 16:1716:17, 22 November 2010 diff hist +941 N Designing siRNA for Knockdown wrote initial page

17 November 2010

• 21:1721:17, 17 November 2010 diff hist +36 Main Page →Protein Quantification

5 November 2010

• 15:0215:02, 5 November 2010 diff hist +29 m Glucose Incorporation into Lipid and Glycogen added new category
• 15:0215:02, 5 November 2010 diff hist −69 m Acetate Incorporation into Lipid removed duplicate listing
• 14:5614:56, 5 November 2010 diff hist −1,040 Acetate Incorporation into Lipid updated with detailed protocol

2 November 2010

• 13:0113:01, 2 November 2010 diff hist +1,705 N Liposome Binding Assay migrated liposome binding protocol current

1 November 2010

• 14:1914:19, 1 November 2010 diff hist −2 Insulin Tolerance Test updated insulin concentration

14 October 2010

• 13:0613:06, 14 October 2010 diff hist +27 Real Time PCR From Cell Culture added categories

13 October 2010

• 18:4618:46, 13 October 2010 diff hist +85 Preparation of Protein Lysates from Mouse Tissues added categories
• 18:4518:45, 13 October 2010 diff hist +128 Preparation of RNA Samples from Mouse Tissues added categories

11 October 2010

• 14:3514:35, 11 October 2010 diff hist +69 Acetate Incorporation into Lipid added radioactive acetate to materials
• 14:2314:23, 11 October 2010 diff hist +1,495 Acetate Incorporation into Lipid added more notes
• 14:2314:23, 11 October 2010 diff hist +2,470 N Talk:Acetate Incorporation into Lipid moved notes to discussion page current