User contributions for Davebrid

18 May 2011

• 20:4520:45, 18 May 2011 diff hist +1,019 N Measurement of Glycolysis and Respiration Using Seahorse wrote initial page current

13 May 2011

• 15:0015:00, 13 May 2011 diff hist +220 Insulin Tolerance Test updated with new starvation and added additional information

20 April 2011

• 15:3115:31, 20 April 2011 diff hist +846 N Analysing Sequencing Results with SeqMan wrote initial page current

14 April 2011

• 14:4014:40, 14 April 2011 diff hist +1 Surface Plasmon Resonance - Protein Lipid Interactions updated protocol to make 300 uL of lipid to make extruding easier current

29 March 2011

• 00:4200:42, 29 March 2011 diff hist +107 Real Time PCR From Cell Culture added reference for calculating amounts from Ct current

16 March 2011

• 16:5316:53, 16 March 2011 diff hist +318 Colocalization updated protocol current

9 March 2011

• 19:5519:55, 9 March 2011 diff hist +720 N Lipid Transfection Created page with '==Materials== * Shuttle Kit from Echelon [http://www.echelon-inc.com/commerce/ccc1021-shuttle-pip-kits.htm] * I used C8 lipids and dissolved to 1 mM in PBS and frozen in small al...' current

1 March 2011

• 21:3321:33, 1 March 2011 diff hist −2 Acetate Incorporation into Lipid increased from 0.1 to 10 uCi per mL.

9 February 2011

• 19:4719:47, 9 February 2011 diff hist +177 Affinity Purification of Proteins - FPLC reworded some bits of the protocol current

2 February 2011

• 19:0419:04, 2 February 2011 diff hist +31 m Immunofluoresence added categories
• 19:0319:03, 2 February 2011 diff hist +57 N Category:Yeast Created page with 'Protocols using yeast, typically cell biology experiments' current

31 January 2011

• 20:5520:55, 31 January 2011 diff hist +73 Protein-Lipid Overlay Assay added categories current
• 20:5420:54, 31 January 2011 diff hist +1,147 N Protein-Lipid Overlay Assay copied protocol

12 January 2011

• 19:4619:46, 12 January 2011 diff hist +94 m Glucose Uptake Assay added categories

13 December 2010

• 19:4719:47, 13 December 2010 diff hist 0 Preparing an Adenoviral shRNA Clone →Choosing a shRNA Sequence: updated XbaI compatible overhang current

10 December 2010

• 21:4921:49, 10 December 2010 diff hist +33 Real Time PCR From Cell Culture altered primer concentration and volumes for 384 well plate
• 20:2320:23, 10 December 2010 diff hist +82 m Glucose Tolerance Test added categories

6 December 2010

• 17:1517:15, 6 December 2010 diff hist +53 Real Time PCR From Cell Culture added link to preparation of rna from tissue
• 15:0715:07, 6 December 2010 diff hist −70 m Genotyping Program No edit summary
• 15:0615:06, 6 December 2010 diff hist +497 N Genotyping Program Created page with '{| border="1" !| Allele || Program|| Fragment Size(s) || Notes |- |TSC-floxed || Inoki PCR || || Two bands if heterozygote |- |Cre || Inoki PCR || || Only present if Transgene...'
• 14:5014:50, 6 December 2010 diff hist +165 GoTaq PCR Genotyping updated protocol and added categories current

2 December 2010

• 22:0022:00, 2 December 2010 diff hist +2,189 N Purification of MBP Fusion Proteins wrote initial page current

22 November 2010

• 16:1716:17, 22 November 2010 diff hist +941 N Designing siRNA for Knockdown wrote initial page

17 November 2010

• 21:1721:17, 17 November 2010 diff hist +36 Main Page →Protein Quantification

5 November 2010

• 15:0215:02, 5 November 2010 diff hist +29 m Glucose Incorporation into Lipid and Glycogen added new category
• 15:0215:02, 5 November 2010 diff hist −69 m Acetate Incorporation into Lipid removed duplicate listing
• 14:5614:56, 5 November 2010 diff hist −1,040 Acetate Incorporation into Lipid updated with detailed protocol

2 November 2010

• 13:0113:01, 2 November 2010 diff hist +1,705 N Liposome Binding Assay migrated liposome binding protocol current

1 November 2010

• 14:1914:19, 1 November 2010 diff hist −2 Insulin Tolerance Test updated insulin concentration

14 October 2010

• 13:0613:06, 14 October 2010 diff hist +27 Real Time PCR From Cell Culture added categories

13 October 2010

• 18:4618:46, 13 October 2010 diff hist +85 Preparation of Protein Lysates from Mouse Tissues added categories
• 18:4518:45, 13 October 2010 diff hist +128 Preparation of RNA Samples from Mouse Tissues added categories

11 October 2010

• 14:3514:35, 11 October 2010 diff hist +69 Acetate Incorporation into Lipid added radioactive acetate to materials
• 14:2314:23, 11 October 2010 diff hist +1,495 Acetate Incorporation into Lipid added more notes
• 14:2314:23, 11 October 2010 diff hist +2,470 N Talk:Acetate Incorporation into Lipid moved notes to discussion page current

6 October 2010

• 13:1713:17, 6 October 2010 diff hist +66 Glycogen Determination from Cells added cell lysis as per alan cheng

5 October 2010

• 18:2318:23, 5 October 2010 diff hist +39 N Category:Metabolism Created page with 'Protocols related to primary metabolism' current
• 18:2318:23, 5 October 2010 diff hist +41 N Category:Glycogen Created page with 'Protocols related to glycogen metabolism.' current
• 18:2218:22, 5 October 2010 diff hist +75 Glucose Incorporation into Lipid and Glycogen added categories
• 18:2218:22, 5 October 2010 diff hist +75 Glycogen Synthase Assay added categories
• 18:2118:21, 5 October 2010 diff hist +92 Glycogen Determination from Tissues added categories
• 18:2118:21, 5 October 2010 diff hist +1,120 N Glycogen Determination from Cells wrote initial page
• 14:5414:54, 5 October 2010 diff hist +1,018 N Acetate Incorporation into Lipid filled in brief protocol

4 October 2010

• 19:0319:03, 4 October 2010 diff hist +288 N Analysis of FRET Images wrote imagej section current

16 September 2010

• 14:3414:34, 16 September 2010 diff hist +139 Preparing an Adenoviral shRNA Clone added categories
• 14:3214:32, 16 September 2010 diff hist +264 N Acid Etching Coverslips Created page with '==Materials== *Coverslips (25CIRCLE 1.5cm) *1N HCl (make fresh) ==Protocol== #Wash coverslips overnight in 1N HCl #Rinse extensively with water #Wash overnight with water #Autoc...' current
• 14:1814:18, 16 September 2010 diff hist +548 N Rab5 FRET Assay wrote initial protocol current
• 14:0114:01, 16 September 2010 diff hist +37 GoTaq PCR Genotyping edited primer concentration

14 September 2010

• 20:0220:02, 14 September 2010 diff hist +2,975 Generating and Amplifying Adenoviral Constructs changed beckman tube order number
• 18:5318:53, 14 September 2010 diff hist +2,032 N Generating and Amplifying Adenoviral Constructs Added linearlization and transformation protocols