User contributions for Davebrid

14 September 2010

• 14:3714:37, 14 September 2010 diff hist +116 Preparing an Adenoviral shRNA Clone added protocol links

13 September 2010

• 18:0418:04, 13 September 2010 diff hist +805 Affinity Purification of Proteins - FPLC added details from new run
• 13:4713:47, 13 September 2010 diff hist +2,226 N Affinity Purification of Proteins - FPLC wrote initial page

2 September 2010

• 19:4919:49, 2 September 2010 diff hist −2 m Insulin Tolerance Test fixed some formatting errors

1 September 2010

• 18:4718:47, 1 September 2010 diff hist +920 N Measuring Phagocytosis using pHrodo Bioparticles wrote initial protocol current

26 August 2010

• 13:4013:40, 26 August 2010 diff hist +10 m Preparing an Adenoviral shRNA Clone no table of contents
• 13:4013:40, 26 August 2010 diff hist +1,353 Preparing an Adenoviral shRNA Clone filled in protocol from http://www.oligoengine.com/products/psuper/documentation/protocols/pSUPER_protocol.pdf

24 August 2010

• 20:0720:07, 24 August 2010 diff hist +128 Glucose Incorporation into Lipid and Glycogen →Protocol: updated volumes for extraction and added a note about using more hot glucose for non-adipocyte lines

23 August 2010

• 18:2118:21, 23 August 2010 diff hist +102 Preparing an Adenoviral shRNA Clone No edit summary
• 18:1718:17, 23 August 2010 diff hist +638 N Preparing an Adenoviral shRNA Clone Created page with '==Choosing a shRNA Sequence== Use oligoengine to pick a sequence, for example <span style="color:#FF0000">GATC</span><span style="color:#00FFFF">CCC</span>GAATACCGCAATGCCTTAG<spa...'

3 August 2010

• 13:2713:27, 3 August 2010 diff hist +111 GST Pulldown Assay added note about pulldown time

30 July 2010

• 13:2613:26, 30 July 2010 diff hist +3,180 N GST Pulldown Assay wrote initial protocol

21 July 2010

• 19:3919:39, 21 July 2010 diff hist +628 N Using South To Migrate a Django Database wrote initial instructions current

18 July 2010

• 23:3623:36, 18 July 2010 diff hist +3,209 N Yeast Invertase Assay copied page from PMID 10564282 current

9 July 2010

• 17:1317:13, 9 July 2010 diff hist −22 m Talk:Colocalization formatting changes current
• 17:1217:12, 9 July 2010 diff hist +1,598 N Talk:Colocalization added notes from the AOMF seminar on colocalization

28 June 2010

• 18:1618:16, 28 June 2010 diff hist +19 N GST-EEA1 Pulldown from Yeast Created page with 'enter protocol here'

24 June 2010

• 13:1613:16, 24 June 2010 diff hist +457 Yeast Sch9 Phosphorylation Assay No edit summary current

21 June 2010

• 13:2813:28, 21 June 2010 diff hist −2 m Inositol Labelling of Yeast Cells No edit summary current

18 June 2010

• 17:5217:52, 18 June 2010 diff hist +73 Inositol Labelling of Yeast Cells added categories
• 15:2615:26, 18 June 2010 diff hist +310 Inositol Labelling of Yeast Cells formatted the reagents
• 15:1215:12, 18 June 2010 diff hist −122 Inositol Labelling of Yeast Cells fixed some formatting errors
• 14:5514:55, 18 June 2010 diff hist +7,623 N Inositol Labelling of Yeast Cells copied protocol from sergey

16 June 2010

• 14:4314:43, 16 June 2010 diff hist +2,013 N Immobilizing a Protein on a CM5 Chip for SPR wrote initial page current

11 June 2010

• 13:4213:42, 11 June 2010 diff hist +752 N Quantification of Vacuolar Colocalization wrote initial page current

24 May 2010

• 20:1620:16, 24 May 2010 diff hist +1,466 N TAP Purification of Yeast Extracts wrote initial protocol from Seraphin lab page current

14 May 2010

• 19:2319:23, 14 May 2010 diff hist +10 m HPLC - Plumbing Setup removed TOC current
• 19:2219:22, 14 May 2010 diff hist +2,357 HPLC - Designing a Method for a Sample or Series filled in details for method design current
• 18:3918:39, 14 May 2010 diff hist +48 HPLC - Designing a Method for a Sample or Series left space for notes
• 18:3818:38, 14 May 2010 diff hist +11 HPLC - Running a Sample or Series removed TOC current
• 18:3818:38, 14 May 2010 diff hist +2,629 N HPLC - Running a Sample or Series wrote initial instructions
• 17:2017:20, 14 May 2010 diff hist +610 N HPLC - Designing a Method for a Sample or Series wrote part of initial page
• 16:2916:29, 14 May 2010 diff hist +4,096 N HPLC - Plumbing Setup wrote initial instructions

11 May 2010

• 15:3415:34, 11 May 2010 diff hist +2,751 Surface Plasmon Resonance - Protein Lipid Interactions Updated protocol with more detailed notes.

6 May 2010

• 16:5716:57, 6 May 2010 diff hist +376 Glycogen Determination from Tissues added glucose quantification protocol
• 15:0115:01, 6 May 2010 diff hist +733 N Glycogen Determination from Tissues Wrote initial protocol

28 April 2010

• 14:0514:05, 28 April 2010 diff hist +122 3T3-L1 Adipocyte Fractionation modified volumes to reflect whether using optiprep or not.

27 April 2010

• 15:2415:24, 27 April 2010 diff hist +130 Glycogen Synthase Assay clarified some points

26 April 2010

• 16:4516:45, 26 April 2010 diff hist +813 N Using Git wrote initial page current

23 April 2010

• 13:4213:42, 23 April 2010 diff hist −10 m Bradford Assay formatting change for PMID

30 March 2010

• 20:1120:11, 30 March 2010 diff hist +76 Main Page →Protein Analysis: added links to Glycogen Synthase and Triglyceride Assays
• 20:1120:11, 30 March 2010 diff hist +847 N Triglyceride Assay from Cells and Tissues wrote initial page
• 19:4919:49, 30 March 2010 diff hist +1,289 N Glycogen Synthase Assay wrote initial page

8 March 2010

• 16:1416:14, 8 March 2010 diff hist +24 Cesium Chloride Preparation of DNA added concentration to Acetate Buffer

24 February 2010

• 16:0816:08, 24 February 2010 diff hist +74 Glucose Incorporation into Lipid and Glycogen added details about protein assay
• 16:0716:07, 24 February 2010 diff hist +2,047 Glucose Incorporation into Lipid and Glycogen filled in details from Brady protocol.

17 February 2010

• 18:4518:45, 17 February 2010 diff hist +525 Electroporation of 3T3-L1 Adipocytes changed default siRNA to 5 uL (500 pmoles) and made some extra notes current

8 February 2010

• 21:0121:01, 8 February 2010 diff hist +27 N Glucose Incorporation into Lipid and Glycogen initial skeleton

2 February 2010

• 17:0417:04, 2 February 2010 diff hist +11 m Yeast Sch9 Phosphorylation Assay No edit summary
• 16:5316:53, 2 February 2010 diff hist −1,595 Yeast Sch9 Phosphorylation Assay modified to a protocol