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A user with 953 edits. Account created on 2 August 2025.

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15:0115:01, 6 May 2010 diff hist +733 N Glycogen Determination from Tissues Wrote initial protocol

14:0514:05, 28 April 2010 diff hist +122 3T3-L1 Adipocyte Fractionation modified volumes to reflect whether using optiprep or not.

15:2415:24, 27 April 2010 diff hist +130 Glycogen Synthase Assay clarified some points

16:4516:45, 26 April 2010 diff hist +813 N Using Git wrote initial page current

13:4213:42, 23 April 2010 diff hist −10 m Bradford Assay formatting change for PMID

20:1120:11, 30 March 2010 diff hist +76 Main Page →Protein Analysis: added links to Glycogen Synthase and Triglyceride Assays
20:1120:11, 30 March 2010 diff hist +847 N Triglyceride Assay from Cells and Tissues wrote initial page
19:4919:49, 30 March 2010 diff hist +1,289 N Glycogen Synthase Assay wrote initial page

16:1416:14, 8 March 2010 diff hist +24 Cesium Chloride Preparation of DNA added concentration to Acetate Buffer

16:0816:08, 24 February 2010 diff hist +74 Glucose Incorporation into Lipid and Glycogen added details about protein assay
16:0716:07, 24 February 2010 diff hist +2,047 Glucose Incorporation into Lipid and Glycogen filled in details from Brady protocol.

18:4518:45, 17 February 2010 diff hist +525 Electroporation of 3T3-L1 Adipocytes changed default siRNA to 5 uL (500 pmoles) and made some extra notes current

21:0121:01, 8 February 2010 diff hist +27 N Glucose Incorporation into Lipid and Glycogen initial skeleton

17:0417:04, 2 February 2010 diff hist +11 m Yeast Sch9 Phosphorylation Assay No edit summary
16:5316:53, 2 February 2010 diff hist −1,595 Yeast Sch9 Phosphorylation Assay modified to a protocol

15:2415:24, 27 January 2010 diff hist −3 FM4-64 Labeling of Yeast Cells updated synaptored concentration current

16:2616:26, 20 January 2010 diff hist +69 FM4-64 Labeling of Yeast Cells Changed dye concentration to match klionsky protocol

16:2216:22, 15 January 2010 diff hist −581 Yeast Sch9 Phosphorylation Assay No edit summary
16:1716:17, 15 January 2010 diff hist −2 Yeast Sch9 Phosphorylation Assay No edit summary
16:1616:16, 15 January 2010 diff hist +3,244 N Yeast Sch9 Phosphorylation Assay Created page with 'from PMID 17560372 Kinase Assay TORC1 was purified from RL175-2d or RL176-1b cells treated with drug vehicle or 200 nM rapamycin for 30 min. Cells grown at 30°C in YPD (250 ...'

15:1515:15, 13 January 2010 diff hist +178 Mutagenesis updated for new PCR program and to use Pfu Turbo as the polymerase current

17:1417:14, 22 December 2009 diff hist +1 m Yeast Transformation typo with dna volume current
16:4816:48, 22 December 2009 diff hist 0 m Yeast Transformation table formatting error
15:4915:49, 22 December 2009 diff hist +24 m Connect to Network Drives No edit summary current
15:4715:47, 22 December 2009 diff hist 0 m Connect to Network Drives No edit summary
15:4715:47, 22 December 2009 diff hist +543 N Connect to Network Drives wrote initial page

17:5617:56, 21 December 2009 diff hist +1 m Yeast Transformation No edit summary
17:5617:56, 21 December 2009 diff hist +63 Yeast Transformation added link to agar plates
17:5417:54, 21 December 2009 diff hist +1,913 N Yeast Transformation wrote initial page
15:2815:28, 21 December 2009 diff hist +70 RIPA Buffer changed vanadate concentration and volume

16:3916:39, 18 December 2009 diff hist 0 m Lipofectamine Mediated Knockdown fixed typo pmoles should be nmoles current
16:3816:38, 18 December 2009 diff hist −2,706 Lipofectamine Mediated Knockdown rewrote according to lipofectamine instructions

14:1014:10, 15 December 2009 diff hist +12 m Main Page added bullet points
14:0914:09, 15 December 2009 diff hist +417 Main Page added media and buffers to index page
14:0214:02, 15 December 2009 diff hist +1,248 N Yeast Selective Media and Agar wrote initial page

15:0215:02, 14 December 2009 diff hist −54 m Yeast Transfection (Small Scale) formatting changes current
15:0115:01, 14 December 2009 diff hist +1,688 N Yeast Transfection (Small Scale) initial copy from protocol folder

19:5119:51, 7 December 2009 diff hist +54 m Main Page No edit summary
19:4819:48, 7 December 2009 diff hist +43 m FM4-64 Labeling of Yeast Cells No edit summary
19:4019:40, 7 December 2009 diff hist +56 FM4-64 Labeling of Yeast Cells added SynaptoRed concentration
16:4716:47, 7 December 2009 diff hist +2 m FM4-64 Labeling of Yeast Cells No edit summary
16:4716:47, 7 December 2009 diff hist +453 FM4-64 Labeling of Yeast Cells added details from Emily

14:1814:18, 30 November 2009 diff hist +168 Lipofectamine Plasmid Transfection No edit summary

15:4615:46, 18 November 2009 diff hist +57 m Generating and Displaying an Electostatic Surface No edit summary current
15:3815:38, 18 November 2009 diff hist +177 Generating and Displaying an Electostatic Surface No edit summary

17:1317:13, 4 November 2009 diff hist +10 m Purification of GST-HA-S6K No edit summary
15:3215:32, 4 November 2009 diff hist +82 m Purification of GST-HA-S6K No edit summary
15:3215:32, 4 November 2009 diff hist +1,071 N Purification of GST-HA-S6K initial page
14:5014:50, 4 November 2009 diff hist −5 HNTG Buffer No edit summary current
14:4914:49, 4 November 2009 diff hist +368 N HNTG Buffer Created page with 'Category:Buffer Category:Immunoprecipitation {| border="1" ! || Final Concentration || per 10 mL || Stock || Location |- | HEPES, pH 7.4 || 50 mM || 500 uL || 1M || 4C...'

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