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A user with 938 edits. Account created on 2 August 2025.

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15:2415:24, 27 January 2010 diff hist −3 FM4-64 Labeling of Yeast Cells updated synaptored concentration current

16:2616:26, 20 January 2010 diff hist +69 FM4-64 Labeling of Yeast Cells Changed dye concentration to match klionsky protocol

16:2216:22, 15 January 2010 diff hist −581 Yeast Sch9 Phosphorylation Assay No edit summary
16:1716:17, 15 January 2010 diff hist −2 Yeast Sch9 Phosphorylation Assay No edit summary
16:1616:16, 15 January 2010 diff hist +3,244 N Yeast Sch9 Phosphorylation Assay Created page with 'from PMID 17560372 Kinase Assay TORC1 was purified from RL175-2d or RL176-1b cells treated with drug vehicle or 200 nM rapamycin for 30 min. Cells grown at 30°C in YPD (250 ...'

15:1515:15, 13 January 2010 diff hist +178 Mutagenesis updated for new PCR program and to use Pfu Turbo as the polymerase current

17:1417:14, 22 December 2009 diff hist +1 m Yeast Transformation typo with dna volume current
16:4816:48, 22 December 2009 diff hist 0 m Yeast Transformation table formatting error
15:4915:49, 22 December 2009 diff hist +24 m Connect to Network Drives No edit summary current
15:4715:47, 22 December 2009 diff hist 0 m Connect to Network Drives No edit summary
15:4715:47, 22 December 2009 diff hist +543 N Connect to Network Drives wrote initial page

17:5617:56, 21 December 2009 diff hist +1 m Yeast Transformation No edit summary
17:5617:56, 21 December 2009 diff hist +63 Yeast Transformation added link to agar plates
17:5417:54, 21 December 2009 diff hist +1,913 N Yeast Transformation wrote initial page
15:2815:28, 21 December 2009 diff hist +70 RIPA Buffer changed vanadate concentration and volume

16:3916:39, 18 December 2009 diff hist 0 m Lipofectamine Mediated Knockdown fixed typo pmoles should be nmoles current
16:3816:38, 18 December 2009 diff hist −2,706 Lipofectamine Mediated Knockdown rewrote according to lipofectamine instructions

14:1014:10, 15 December 2009 diff hist +12 m Main Page added bullet points
14:0914:09, 15 December 2009 diff hist +417 Main Page added media and buffers to index page
14:0214:02, 15 December 2009 diff hist +1,248 N Yeast Selective Media and Agar wrote initial page

15:0215:02, 14 December 2009 diff hist −54 m Yeast Transfection (Small Scale) formatting changes current
15:0115:01, 14 December 2009 diff hist +1,688 N Yeast Transfection (Small Scale) initial copy from protocol folder

19:5119:51, 7 December 2009 diff hist +54 m Main Page No edit summary
19:4819:48, 7 December 2009 diff hist +43 m FM4-64 Labeling of Yeast Cells No edit summary
19:4019:40, 7 December 2009 diff hist +56 FM4-64 Labeling of Yeast Cells added SynaptoRed concentration
16:4716:47, 7 December 2009 diff hist +2 m FM4-64 Labeling of Yeast Cells No edit summary
16:4716:47, 7 December 2009 diff hist +453 FM4-64 Labeling of Yeast Cells added details from Emily

14:1814:18, 30 November 2009 diff hist +168 Lipofectamine Plasmid Transfection No edit summary

15:4615:46, 18 November 2009 diff hist +57 m Generating and Displaying an Electostatic Surface No edit summary current
15:3815:38, 18 November 2009 diff hist +177 Generating and Displaying an Electostatic Surface No edit summary

17:1317:13, 4 November 2009 diff hist +10 m Purification of GST-HA-S6K No edit summary
15:3215:32, 4 November 2009 diff hist +82 m Purification of GST-HA-S6K No edit summary
15:3215:32, 4 November 2009 diff hist +1,071 N Purification of GST-HA-S6K initial page
14:5014:50, 4 November 2009 diff hist −5 HNTG Buffer No edit summary current
14:4914:49, 4 November 2009 diff hist +368 N HNTG Buffer Created page with 'Category:Buffer Category:Immunoprecipitation {| border="1" ! || Final Concentration || per 10 mL || Stock || Location |- | HEPES, pH 7.4 || 50 mM || 500 uL || 1M || 4C...'

21:2421:24, 3 November 2009 diff hist −75 PCR Amplification of DNA (KOD Polymerase) No edit summary current
21:2421:24, 3 November 2009 diff hist −14 m PCR Amplification of DNA (KOD Polymerase) No edit summary
21:2321:23, 3 November 2009 diff hist +1,258 Nm PCR Amplification of DNA (KOD Polymerase) initial page

16:2716:27, 27 October 2009 diff hist +102 m Preparation of DIG Labelled Probes No edit summary current
16:2716:27, 27 October 2009 diff hist +40 m Main Page →Protein Analysis
16:2616:26, 27 October 2009 diff hist +921 N Preparation of DIG Labelled Probes wrote initial page

15:4315:43, 21 October 2009 diff hist +82 m Colony PCR No edit summary current
15:4315:43, 21 October 2009 diff hist +17 m Main Page →Genotyping
15:4215:42, 21 October 2009 diff hist +608 N Colony PCR Created page with '==Reagents== *Primers *dNTPs (2 mM of each dNTP) *Expand polymerase (Roche) *Primers *Yeast Colonies ==Protocol== #Pick about 20% of a colony and mix in 20 uL of sterile water. ...'

14:5014:50, 20 October 2009 diff hist +2 Differentiation of 3T3-L1 Cells No edit summary

18:3018:30, 14 October 2009 diff hist +11 m Glucose Uptake Assay No edit summary
18:2718:27, 14 October 2009 diff hist +455 N KRBH Buffer initial page current
18:2118:21, 14 October 2009 diff hist +277 Rapamycin Sensitivity Assay of Yeast added notes about dilutions and incubations current
18:1918:19, 14 October 2009 diff hist +11 Glucose Uptake Assay added link to KRBH Buffer

15:0515:05, 13 October 2009 diff hist +57 m Rapamycin Sensitivity Assay of Yeast No edit summary

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