User contributions for Davebrid
From Bridges Lab Protocols
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3 November 2009
- 21:2421:24, 3 November 2009 diff hist −75 PCR Amplification of DNA (KOD Polymerase) No edit summary current
- 21:2421:24, 3 November 2009 diff hist −14 m PCR Amplification of DNA (KOD Polymerase) No edit summary
- 21:2321:23, 3 November 2009 diff hist +1,258 Nm PCR Amplification of DNA (KOD Polymerase) initial page
27 October 2009
- 16:2716:27, 27 October 2009 diff hist +102 m Preparation of DIG Labelled Probes No edit summary current
- 16:2716:27, 27 October 2009 diff hist +40 m Main Page →Protein Analysis
- 16:2616:26, 27 October 2009 diff hist +921 N Preparation of DIG Labelled Probes wrote initial page
21 October 2009
- 15:4315:43, 21 October 2009 diff hist +82 m Colony PCR No edit summary current
- 15:4315:43, 21 October 2009 diff hist +17 m Main Page →Genotyping
- 15:4215:42, 21 October 2009 diff hist +608 N Colony PCR Created page with '==Reagents== *Primers *dNTPs (2 mM of each dNTP) *Expand polymerase (Roche) *Primers *Yeast Colonies ==Protocol== #Pick about 20% of a colony and mix in 20 uL of sterile water. ...'
20 October 2009
- 14:5014:50, 20 October 2009 diff hist +2 Differentiation of 3T3-L1 Cells No edit summary
14 October 2009
- 18:3018:30, 14 October 2009 diff hist +11 m Glucose Uptake Assay No edit summary
- 18:2718:27, 14 October 2009 diff hist +455 N KRBH Buffer initial page current
- 18:2118:21, 14 October 2009 diff hist +277 Rapamycin Sensitivity Assay of Yeast added notes about dilutions and incubations current
- 18:1918:19, 14 October 2009 diff hist +11 Glucose Uptake Assay added link to KRBH Buffer
13 October 2009
- 15:0515:05, 13 October 2009 diff hist +57 m Rapamycin Sensitivity Assay of Yeast No edit summary
- 13:2813:28, 13 October 2009 diff hist +578 N Rapamycin Sensitivity Assay of Yeast Created page with '==Materials== *YPD Media *YPD Plates with either DMSO or 2-200 nM Rapamycin *Yeast strains, streaked out on appropriate plate (< 1 month old) ==Protocol== #Innoculate 3 mL of YP...'
10 October 2009
- 00:5300:53, 10 October 2009 diff hist +2 m Generating and Displaying an Electostatic Surface No edit summary
- 00:5200:52, 10 October 2009 diff hist +6 m Generating and Displaying an Electostatic Surface No edit summary
- 00:5200:52, 10 October 2009 diff hist +376 m Generating and Displaying an Electostatic Surface No edit summary
- 00:3900:39, 10 October 2009 diff hist +76 m Generating and Displaying an Electostatic Surface No edit summary
- 00:3800:38, 10 October 2009 diff hist +72 m Generating and Displaying an Electostatic Surface →Generate PQR File for the Structure
- 00:2000:20, 10 October 2009 diff hist +260 N Generating and Displaying an Electostatic Surface Created page with '==Programs Required== *Pymol *APBS ===Generate PQR File for the Structure=== #Download or generate PDB file. #Go to the PDB2PQR server at http://pdb2pqr-1.wustl.edu/pdb2pqr/. T...'
9 October 2009
- 12:5712:57, 9 October 2009 diff hist +41 YPD Media and Agar No edit summary
- 12:5712:57, 9 October 2009 diff hist +521 N YPD Media and Agar Created page with '==Materials== *YPD Medium (Clontech 630409) *Agar (if making plates) *Adenine Hemisulfate if making YPDA. Dissolve to 0.2% and autoclave. ==Preparation== *Weigh out 5g/100 mL o...'
5 October 2009
- 19:5719:57, 5 October 2009 diff hist +276 N FM4-64 Labeling of Yeast Cells initial page
1 October 2009
- 14:3114:31, 1 October 2009 diff hist +41 m Designing and Preparing dsRNA No edit summary current
- 14:3114:31, 1 October 2009 diff hist +1,236 N Using ImageJ to Quantify Bands updated and clarified some points current
29 September 2009
- 20:1420:14, 29 September 2009 diff hist −26 m DsRNA Mediated Knockdown of S2 Cells No edit summary current
- 20:1320:13, 29 September 2009 diff hist +23 m DsRNA Mediated Knockdown of S2 Cells No edit summary
- 20:1120:11, 29 September 2009 diff hist −113 m DsRNA Mediated Knockdown of S2 Cells No edit summary
- 20:1120:11, 29 September 2009 diff hist +113 m DsRNA Mediated Knockdown of S2 Cells No edit summary
- 20:0720:07, 29 September 2009 diff hist +46 m DsRNA Mediated Knockdown of S2 Cells No edit summary
- 20:0720:07, 29 September 2009 diff hist +17 m DsRNA Mediated Knockdown of S2 Cells No edit summary
- 20:0520:05, 29 September 2009 diff hist +190 m DsRNA Mediated Knockdown of S2 Cells No edit summary
- 20:0320:03, 29 September 2009 diff hist −20 m DsRNA Mediated Knockdown of S2 Cells No edit summary
- 20:0020:00, 29 September 2009 diff hist +809 N DsRNA Mediated Knockdown of S2 Cells initial page
16 September 2009
- 15:4015:40, 16 September 2009 diff hist +50 m Designing and Preparing dsRNA →Preparing dsRNA
- 15:3815:38, 16 September 2009 diff hist +5 m Designing and Preparing dsRNA No edit summary
14 September 2009
- 19:3619:36, 14 September 2009 diff hist +110 m Designing and Preparing dsRNA No edit summary
- 19:3419:34, 14 September 2009 diff hist +83 m Designing and Preparing dsRNA No edit summary
- 19:3319:33, 14 September 2009 diff hist +1,338 Designing and Preparing dsRNA added details about dsRNA preparation
13 September 2009
- 14:4214:42, 13 September 2009 diff hist +49 Using Mr Bayes to For Phlyogenetic Analysis changed instructions for burnin current
10 September 2009
- 18:5518:55, 10 September 2009 diff hist +10 m 3T3-L1 Adipocyte Fractionation No edit summary
- 18:4618:46, 10 September 2009 diff hist −12 m 3T3-L1 Adipocyte Fractionation →References
- 12:4212:42, 10 September 2009 diff hist 0 m Using Mr Bayes to For Phlyogenetic Analysis No edit summary
9 September 2009
- 19:3519:35, 9 September 2009 diff hist +70 m Using Mr Bayes to For Phlyogenetic Analysis No edit summary
- 19:2219:22, 9 September 2009 diff hist +1,092 N Using Mr Bayes to For Phlyogenetic Analysis wrote initial page
2 September 2009
- 15:3515:35, 2 September 2009 diff hist +12 m Generation of Heatmaps from Microarray Data (KEGG2heatmap or GO2heatmap) No edit summary current
- 15:3415:34, 2 September 2009 diff hist +27 m Using Bioconductor To Analyse Microarray Data No edit summary current
- 15:3415:34, 2 September 2009 diff hist +27 m Using Bioconductor To Analyse Beadarray Data (lumi) No edit summary current