User contributions for Davebrid

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A user with 938 edits. Account created on 2 August 2025.

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13:2813:28, 13 October 2009 diff hist +578 N Rapamycin Sensitivity Assay of Yeast Created page with '==Materials== *YPD Media *YPD Plates with either DMSO or 2-200 nM Rapamycin *Yeast strains, streaked out on appropriate plate (< 1 month old) ==Protocol== #Innoculate 3 mL of YP...'

00:5300:53, 10 October 2009 diff hist +2 m Generating and Displaying an Electostatic Surface No edit summary
00:5200:52, 10 October 2009 diff hist +6 m Generating and Displaying an Electostatic Surface No edit summary
00:5200:52, 10 October 2009 diff hist +376 m Generating and Displaying an Electostatic Surface No edit summary
00:3900:39, 10 October 2009 diff hist +76 m Generating and Displaying an Electostatic Surface No edit summary
00:3800:38, 10 October 2009 diff hist +72 m Generating and Displaying an Electostatic Surface →Generate PQR File for the Structure
00:2000:20, 10 October 2009 diff hist +260 N Generating and Displaying an Electostatic Surface Created page with '==Programs Required== *Pymol *APBS ===Generate PQR File for the Structure=== #Download or generate PDB file. #Go to the PDB2PQR server at http://pdb2pqr-1.wustl.edu/pdb2pqr/. T...'

12:5712:57, 9 October 2009 diff hist +41 YPD Media and Agar No edit summary
12:5712:57, 9 October 2009 diff hist +521 N YPD Media and Agar Created page with '==Materials== *YPD Medium (Clontech 630409) *Agar (if making plates) *Adenine Hemisulfate if making YPDA. Dissolve to 0.2% and autoclave. ==Preparation== *Weigh out 5g/100 mL o...'

19:5719:57, 5 October 2009 diff hist +276 N FM4-64 Labeling of Yeast Cells initial page

14:3114:31, 1 October 2009 diff hist +41 m Designing and Preparing dsRNA No edit summary current
14:3114:31, 1 October 2009 diff hist +1,236 N Using ImageJ to Quantify Bands updated and clarified some points current

20:1420:14, 29 September 2009 diff hist −26 m DsRNA Mediated Knockdown of S2 Cells No edit summary current
20:1320:13, 29 September 2009 diff hist +23 m DsRNA Mediated Knockdown of S2 Cells No edit summary
20:1120:11, 29 September 2009 diff hist −113 m DsRNA Mediated Knockdown of S2 Cells No edit summary
20:1120:11, 29 September 2009 diff hist +113 m DsRNA Mediated Knockdown of S2 Cells No edit summary
20:0720:07, 29 September 2009 diff hist +46 m DsRNA Mediated Knockdown of S2 Cells No edit summary
20:0720:07, 29 September 2009 diff hist +17 m DsRNA Mediated Knockdown of S2 Cells No edit summary
20:0520:05, 29 September 2009 diff hist +190 m DsRNA Mediated Knockdown of S2 Cells No edit summary
20:0320:03, 29 September 2009 diff hist −20 m DsRNA Mediated Knockdown of S2 Cells No edit summary
20:0020:00, 29 September 2009 diff hist +809 N DsRNA Mediated Knockdown of S2 Cells initial page

15:4015:40, 16 September 2009 diff hist +50 m Designing and Preparing dsRNA →Preparing dsRNA
15:3815:38, 16 September 2009 diff hist +5 m Designing and Preparing dsRNA No edit summary

19:3619:36, 14 September 2009 diff hist +110 m Designing and Preparing dsRNA No edit summary
19:3419:34, 14 September 2009 diff hist +83 m Designing and Preparing dsRNA No edit summary
19:3319:33, 14 September 2009 diff hist +1,338 Designing and Preparing dsRNA added details about dsRNA preparation

14:4214:42, 13 September 2009 diff hist +49 Using Mr Bayes to For Phlyogenetic Analysis changed instructions for burnin current

18:5518:55, 10 September 2009 diff hist +10 m 3T3-L1 Adipocyte Fractionation No edit summary
18:4618:46, 10 September 2009 diff hist −12 m 3T3-L1 Adipocyte Fractionation →References
12:4212:42, 10 September 2009 diff hist 0 m Using Mr Bayes to For Phlyogenetic Analysis No edit summary

19:3519:35, 9 September 2009 diff hist +70 m Using Mr Bayes to For Phlyogenetic Analysis No edit summary
19:2219:22, 9 September 2009 diff hist +1,092 N Using Mr Bayes to For Phlyogenetic Analysis wrote initial page

15:3515:35, 2 September 2009 diff hist +12 m Generation of Heatmaps from Microarray Data (KEGG2heatmap or GO2heatmap) No edit summary current
15:3415:34, 2 September 2009 diff hist +27 m Using Bioconductor To Analyse Microarray Data No edit summary current
15:3415:34, 2 September 2009 diff hist +27 m Using Bioconductor To Analyse Beadarray Data (lumi) No edit summary current
15:3415:34, 2 September 2009 diff hist +27 m Using Bioconductor To Analyse Beadarray Data No edit summary current
15:3315:33, 2 September 2009 diff hist +69 m Generation of Heatmaps from Microarray Data (KEGG2heatmap or GO2heatmap) No edit summary
15:3215:32, 2 September 2009 diff hist +3,060 N Generation of Heatmaps from Microarray Data (KEGG2heatmap or GO2heatmap) intial page

18:1618:16, 26 August 2009 diff hist +47 m CHAPS Lysis Buffer No edit summary current
18:1518:15, 26 August 2009 diff hist +131 MTORC1 Kinase Assay added substrate and ATP separately current
17:3417:34, 26 August 2009 diff hist +9 m MTORC1 Kinase Assay No edit summary
17:3317:33, 26 August 2009 diff hist +89 m MTORC1 Kinase Assay No edit summary
17:3317:33, 26 August 2009 diff hist +919 N MTORC1 Kinase Assay Created page with '==Materials== *Cells treated as required. *Purified GST-HA-S6K1 (purified from 293T cells) *mTOR antibody (Santa Cruz) *Protein A/G Beads *CHAPS Lysis Buffer *[[mTORC1 Kinase...'
13:5813:58, 26 August 2009 diff hist +397 N TORC1 Kinase Buffer wrote initial page current

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