User contributions for Davebrid

26 May 2009

• 21:2521:25, 26 May 2009 diff hist +123 Preparing Cell Lysates edited phosphatase inhibitors
• 21:1521:15, 26 May 2009 diff hist +2 m Preparing Cell Lysates →RIPA Buffer (for 10mL lysis buffer)
• 21:1421:14, 26 May 2009 diff hist +117 Preparing Cell Lysates edited table
• 20:1220:12, 26 May 2009 diff hist −28 Real Time PCR From Cell Culture →Plate Preparation
• 18:5118:51, 26 May 2009 diff hist +82 Real Time PCR From Cell Culture →RT-PCR Reaction

20 May 2009

• 15:0715:07, 20 May 2009 diff hist +79 Glucose Tolerance Test No edit summary

13 May 2009

• 15:4215:42, 13 May 2009 diff hist +116 Glucose Tolerance Test →Protocol
• 15:4115:41, 13 May 2009 diff hist +21 Glucose Tolerance Test →Materials (Bring Downstairs)
• 13:4313:43, 13 May 2009 diff hist +2 Main Page →Metabolic Measurements
• 13:4313:43, 13 May 2009 diff hist +847 N Glucose Tolerance Test wrote initial page
• 13:3213:32, 13 May 2009 diff hist +55 Main Page →Genotyping
• 13:3113:31, 13 May 2009 diff hist +50 Main Page →Transcriptional Analysis

11 May 2009

• 15:2215:22, 11 May 2009 diff hist −5 Main Page →Cell and Tissue Culture
• 15:2215:22, 11 May 2009 diff hist +33 Main Page →Cell and Tissue Culture
• 15:2115:21, 11 May 2009 diff hist +19 N Preparing Cell Lysates Created page with 'type something here'
• 14:1014:10, 11 May 2009 diff hist +50 N DNA Preparation from Tail Clip Created page with 'Use Qiagen DNEasy kit for Blood and Tissue Samples'
• 14:0914:09, 11 May 2009 diff hist +32 Main Page →Protein Purification
• 14:0914:09, 11 May 2009 diff hist +611 N Determining Percent Purity wrote initial page
• 14:0314:03, 11 May 2009 diff hist 0 Quantification by Absorbance at 280nm No edit summary current
• 14:0214:02, 11 May 2009 diff hist +702 N Quantification by Absorbance at 280nm wrote initial page
• 13:4313:43, 11 May 2009 diff hist −46 Main Page No edit summary

7 May 2009

• 17:4117:41, 7 May 2009 diff hist +17 Electroporation of 3T3-L1 Adipocytes No edit summary
• 17:3917:39, 7 May 2009 diff hist +858 N Electroporation of 3T3-L1 Adipocytes Created page with '==Materials== *Differentiated cells FBS day 3 or less. *Gene Pulser cuvette *DNA: CsCl Purified or siRNA (resuspended to 0.1 nmol/uL with 200 uL water) *PBS +/+ *PBS -/- *0.25% ...'
• 17:2917:29, 7 May 2009 diff hist +42 Main Page →Cell and Tissue Culture
• 14:4014:40, 7 May 2009 diff hist +1 m Restriction Enzyme Based Cloning No edit summary current
• 14:2214:22, 7 May 2009 diff hist +252 Restriction Enzyme Based Cloning updated with gel purification

6 May 2009

• 18:5218:52, 6 May 2009 diff hist +2 PCR Analysis of Tail DNA No edit summary
• 18:5218:52, 6 May 2009 diff hist +2 PCR Analysis of Tail DNA →Protocol
• 18:5218:52, 6 May 2009 diff hist +148 N PCR Analysis of Tail DNA Created page with 'see [Genotyping Details] for strain specific details ==Materials== ==Protocol== see [Preparing an Agarose Gel] for details on preparing a DNA gel'
• 18:5018:50, 6 May 2009 diff hist +139 Main Page No edit summary
• 15:1615:16, 6 May 2009 diff hist +12 m Induction Conditions No edit summary
• 15:1515:15, 6 May 2009 diff hist −54 m Induction Conditions No edit summary
• 15:1515:15, 6 May 2009 diff hist −9 m Induction Conditions No edit summary
• 15:1415:14, 6 May 2009 diff hist +218 N Induction Conditions Created page with '{| class="wikitable" |- ! Protein ! IPTG Concentration ! Time ! Temperature ! Notes |- | some protein | some IPTG | some time | some temperature | some note |- | row 2, cel...'
• 15:1215:12, 6 May 2009 diff hist +30 m Purification of GST Fusion Proteins No edit summary
• 15:0915:09, 6 May 2009 diff hist +21 Main Page →Cell and Tissue Culture
• 15:0815:08, 6 May 2009 diff hist +519 N Splitting Cells Created page with '==Materials== *FBS (L1-FBS for 3T3-L1, COS-FBS for others): Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM *PBS -/- *0.05% Trypsin ==Protocol== #W...'

5 May 2009

• 20:1920:19, 5 May 2009 diff hist +1 m Restriction Enzyme Based Cloning - Ordering Primers →Preparation and Ordering Primers current
• 16:0316:03, 5 May 2009 diff hist +484 N Preparing an Agarose Gel wrote page
• 16:0016:00, 5 May 2009 diff hist +30 Main Page No edit summary
• 15:0815:08, 5 May 2009 diff hist +30 Main Page No edit summary
• 15:0815:08, 5 May 2009 diff hist +41 Main Page No edit summary
• 15:0715:07, 5 May 2009 diff hist +1,767 N Purification of GST Fusion Proteins Created page with '==Bacteria Production and Induction== #Express and induce protein in culture under appropriate conditions (normally do the following) #grow an overnight culture in ~25 mL LB/Amp ...'
• 13:2513:25, 5 May 2009 diff hist 0 m PCR Amplification of DNA →Protocol
• 13:2413:24, 5 May 2009 diff hist +11 m PCR Amplification of DNA →Protocol
• 13:0913:09, 5 May 2009 diff hist −11 m PCR Amplification of DNA No edit summary
• 13:0913:09, 5 May 2009 diff hist +1 PCR Amplification of DNA No edit summary
• 13:0813:08, 5 May 2009 diff hist +11 m PCR Amplification of DNA No edit summary
• 12:5312:53, 5 May 2009 diff hist +941 N PCR Amplification of DNA Created page with '==Materials== *Primers – order from IDT prediluted to 100 mM in TE. Make working solution of 1 uM (100x) in water with both sense and antisense primers combined *dNTPs – dil...'

4 May 2009

• 12:4012:40, 4 May 2009 diff hist −8 3T3-L1 Adipocyte Fractionation →Optiprep Gradient