User contributions for Davebrid

4 May 2009

• 12:3912:39, 4 May 2009 diff hist +36 Main Page →Cell and Tissue Culture
• 12:3812:38, 4 May 2009 diff hist +156 3T3-L1 Adipocyte Fractionation No edit summary
• 12:3512:35, 4 May 2009 diff hist +1,336 N 3T3-L1 Adipocyte Fractionation Created page with '==Materials== *PBS *Lysis Buffer (25 mM HEPES, 250 mM Sucrose, PI tablet) *Optiprep ==Fractionation== #Wash cells twice with ice cold PBS -/- #Scrape 150 mm dish into 4 mL of Ly...'
• 12:2212:22, 4 May 2009 diff hist +1,289 N French Press imported protocol
• 12:2012:20, 4 May 2009 diff hist +45 Main Page No edit summary
• 01:3101:31, 4 May 2009 diff hist −2 Help:Contents No edit summary current
• 01:3001:30, 4 May 2009 diff hist −2 Help:Contents No edit summary
• 01:3001:30, 4 May 2009 diff hist 0 Help:Contents No edit summary
• 01:2801:28, 4 May 2009 diff hist +20 Help:Contents No edit summary
• 01:2601:26, 4 May 2009 diff hist +12 Help:Contents →Editing
• 01:2601:26, 4 May 2009 diff hist +42 Help:Contents No edit summary
• 01:2501:25, 4 May 2009 diff hist +20,399 N Help:Preventing access Created page with '{{languages}} For help customizing user rights, see Manual:User rights. This page contains examples useful for restricting access. Most of the examples need changes to [[Ma...' current
• 01:0301:03, 4 May 2009 diff hist +2 Bradford Assay →Reference
• 01:0301:03, 4 May 2009 diff hist +69 Bradford Assay No edit summary
• 01:0001:00, 4 May 2009 diff hist +26 Help:Contents No edit summary
• 00:5900:59, 4 May 2009 diff hist +21 Help:Contents →Editing
• 00:5700:57, 4 May 2009 diff hist −11 Help:Wikitext examples No edit summary current
• 00:5600:56, 4 May 2009 diff hist +28 Help:Contents No edit summary
• 00:5600:56, 4 May 2009 diff hist +19,408 N Help:Wikitext examples Created page with 'from http://en.wikipedia.org/w/index.php?title=Help:Wikitext_examples For basic information see Help:Editing. == Basic text formatting == You can format the page using Wikit...'
• 00:5200:52, 4 May 2009 diff hist −2 m Restriction Enzyme Based Cloning - Ordering Primers No edit summary
• 00:5200:52, 4 May 2009 diff hist −3 m Restriction Enzyme Based Cloning - Ordering Primers No edit summary
• 00:5100:51, 4 May 2009 diff hist 0 m Restriction Enzyme Based Cloning - Ordering Primers No edit summary
• 00:4900:49, 4 May 2009 diff hist +76 Restriction Enzyme Based Cloning - Ordering Primers added ncbi primer blast

2 May 2009

• 01:0601:06, 2 May 2009 diff hist +41 Differentiation of 3T3-L1 Cells No edit summary
• 01:0001:00, 2 May 2009 diff hist +92 N Bridges Lab Protocols:About Created page with 'This page contains protocols developed by and for use in the laboratory of Dr. Alan Saltiel.'
• 00:5800:58, 2 May 2009 diff hist +58 Main Page No edit summary
• 00:5800:58, 2 May 2009 diff hist +1,476 N Restriction Enzyme Based Cloning - Ordering Primers Created page with '==Preparation and Ordering Primers== #Choose target vector and region of interest in gene #Pick 5' and 3' restriction sites that are present in the vector but NOT in the insert #...'
• 00:5400:54, 2 May 2009 diff hist +32 Western Blotting →Protocol
• 00:5300:53, 2 May 2009 diff hist +4 Western Blotting →Materials
• 00:5100:51, 2 May 2009 diff hist +22 Main Page No edit summary
• 00:5100:51, 2 May 2009 diff hist +1,232 N Western Blotting Created page with '==Materials== *Transfer Buffer (100 mL Methanol, 50 mL 10X Transfer Buffer to final 500 mL) *Transfer Apparatus, either Bio-Rad or Invitrogen ==Protocol== #Run SDS-PAGE gel and ...'
• 00:4500:45, 2 May 2009 diff hist +18 Main Page →Cloning and Molecular Biology
• 00:4500:45, 2 May 2009 diff hist +611 N TOPO Cloning Created page with '#PCR desired insert using Pfu polymerase. Add 1 uL of Taq and mix. Incubate 10min at 72C to add A to 3’ ends. Use immediately #If PCR product is a single band, no need to pu...' current
• 00:4000:40, 2 May 2009 diff hist +60 Main Page No edit summary
• 00:3900:39, 2 May 2009 diff hist +41 Surface Plasmon Resonance - Protein Lipid Interactions No edit summary
• 00:3500:35, 2 May 2009 diff hist +1,640 N Surface Plasmon Resonance - Protein Lipid Interactions Created page with '==Materials== *L1 Sensor Chip (Biacore) *Dioleylphosphatidylcholine (Sigma) dissolved in 1:1 chloroform:methanol with 0.1% HCl *PIPx of interest (Avanti or Echelon) dissolved in ...'
• 00:3100:31, 2 May 2009 diff hist +55 Main Page →Cloning and Molecular Biology
• 00:3100:31, 2 May 2009 diff hist +1,020 N Restriction Enzyme Based Cloning copied over protocol
• 00:2900:29, 2 May 2009 diff hist +13 Mutagenesis →Protocol
• 00:2900:29, 2 May 2009 diff hist 0 Mutagenesis →Protocol
• 00:2800:28, 2 May 2009 diff hist +1,049 N Mutagenesis copied over protocol
• 00:0800:08, 2 May 2009 diff hist +23 Main Page →Cell and Tissue Culture
• 00:0800:08, 2 May 2009 diff hist +1 Immunofluoresence No edit summary
• 00:0700:07, 2 May 2009 diff hist +1,242 N Immunofluoresence copied over protocol

1 May 2009

• 23:5823:58, 1 May 2009 diff hist +2 m Main Page →Transcriptional Analysis
• 23:5723:57, 1 May 2009 diff hist −273 Main Page →Getting started: removed section
• 22:5322:53, 1 May 2009 diff hist +621 N Bradford Assay copied over protocol
• 22:5022:50, 1 May 2009 diff hist +109 Main Page No edit summary
• 22:4822:48, 1 May 2009 diff hist +567 N Transformation of Bacteria copied over protocol
• 22:2622:26, 1 May 2009 diff hist +2,942 N Differentiation of 3T3-L1 Cells copied over page