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  1. Monitoring Food Intake
  2. Mutagenesis
  3. NADH NBT Staining
  4. Nr3c1 Genotyping
  5. Ob/ob Genotyping
  6. Oil red o stain for in vitro adipogenesis
  7. Ordering Barcodes for Mice
  8. Ordering Mice
  9. Ordering Prescriptions-University of Michigan
  10. Osmotic Minipump Implantation
  11. PAS Staining
  12. PCR Amplification of DNA
  13. PCR Amplification of DNA (KOD Polymerase)
  14. PCR Amplification with Platinum Taq High Fidelity
  15. PCR Analysis of Tail DNA
  16. PEI Mediated Plasmid Transfection
  17. Paraffin Embedding of Tissue Samples
  18. Parsing XSQ Files
  19. Performing Drosophila Crosses
  20. Perfusion of Mouse
  21. Phosphate Buffered Saline
  22. Phred Quality Scores
  23. Power Analysis
  24. Ppp1r3c Genotyping
  25. Preparation of Chloroquine Stocks
  26. Preparation of DIG Labelled Probes
  27. Preparation of Mouse Muscle for Cryosectioning
  28. Preparation of Orthovanadate Stocks
  29. Preparation of PEI Stocks
  30. Preparation of Protein Lysates from Cells
  31. Preparation of Protein Lysates from Mouse Tissues
  32. Preparation of RNA Sample from Cell Culture
  33. Preparation of RNA Samples from Mouse Tissues
  34. Preparation of Tail Samples (for Genotyping)
  35. Preparing Cell Lysates
  36. Preparing a SDS-PAGE Gel
  37. Preparing an Adenoviral shRNA Clone
  38. Preparing an Agarose Gel
  39. Preparing and Sending Tissues to Histology Core
  40. Primary Adipocyte Isolation
  41. Primer Design for qPCR
  42. Production of LentiCRISPR Viruses
  43. Protein-Lipid Overlay Assay
  44. Purification of GST-HA-S6K
  45. Purification of GST Fusion Proteins
  46. Purification of GST Fusion Proteins by GSTrap
  47. Purification of MBP Fusion Proteins
  48. Purification of miRNA and mRNA with TRIzol
  49. Pyruvate Tolerance Test
  50. QPCR

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