Genotyping Program

Allele	Program	Fragment Size(s)	Notes
TSC-floxed	Cre PCR		Two bands if heterozygote
Cre	Cre PCR		Only present if Transgene Positive (cannot detect homozygosity at transgene)
Vac14	Cre PCR		Two bands if heterozygote
TC10b	TC10b	KO - 500bp, WT-1kb	Two bands if heterozygote
ob/ob Genotyping	ob/ob	KO - 123bp, WT-104kb, control 191 bp	Two reactions, see ob/ob Genotyping
MTM1	MTM1	Mutant-575, WT-714	Separate Reactions for WT and KO
CAP	CAP		Two bands if heterozygote, upper band for KO
Vinexin	Vinexin