Buffer/RIPA

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Revision as of 13:33, 8 July 2009 by Davebrid (talk | contribs) (mistake with NaF and NaPPi concentrations. Added note about NaVO3 location)

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RIPA Buffer (for 10mL lysis buffer)

	Final Concentration	per 10 mL	Stock	Location
Tris pH7.4	50mM	500uL	1M
Na Deoxycholate	0.25%	250uL	10%
NP-40	1%	1mL	10%
NaCl	150mM	375uL	4M
EDTA	1mM	20uL	0.5M
NaVO3	1mM	100uL	1M	Biochemical Stocks (-20)
NaF	5mM	100uL	500mM
NaPPi	25 mM	1 mL	250mM
Protease Inhibitors		1 mini tablet		4C

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