User contributions for Davebridges

4 May 2009

• 01:2601:26, 4 May 2009 diff hist +12 Help:Contents →Editing
• 01:2601:26, 4 May 2009 diff hist +42 Help:Contents No edit summary
• 01:2501:25, 4 May 2009 diff hist +20,399 N Help:Preventing access Created page with '{{languages}} For help customizing user rights, see Manual:User rights. This page contains examples useful for restricting access. Most of the examples need changes to [[Ma...' current
• 01:0301:03, 4 May 2009 diff hist +2 Bradford Assay →Reference
• 01:0301:03, 4 May 2009 diff hist +69 Bradford Assay No edit summary
• 01:0001:00, 4 May 2009 diff hist +26 Help:Contents No edit summary
• 00:5900:59, 4 May 2009 diff hist +21 Help:Contents →Editing
• 00:5700:57, 4 May 2009 diff hist −11 Help:Wikitext examples No edit summary current
• 00:5600:56, 4 May 2009 diff hist +28 Help:Contents No edit summary
• 00:5600:56, 4 May 2009 diff hist +19,408 N Help:Wikitext examples Created page with 'from http://en.wikipedia.org/w/index.php?title=Help:Wikitext_examples For basic information see Help:Editing. == Basic text formatting == You can format the page using Wikit...'
• 00:5200:52, 4 May 2009 diff hist −2 m Restriction Enzyme Based Cloning - Ordering Primers No edit summary
• 00:5200:52, 4 May 2009 diff hist −3 m Restriction Enzyme Based Cloning - Ordering Primers No edit summary
• 00:5100:51, 4 May 2009 diff hist 0 m Restriction Enzyme Based Cloning - Ordering Primers No edit summary
• 00:4900:49, 4 May 2009 diff hist +76 Restriction Enzyme Based Cloning - Ordering Primers added ncbi primer blast

2 May 2009

• 01:0601:06, 2 May 2009 diff hist +41 Differentiation of 3T3-L1 Cells No edit summary
• 01:0001:00, 2 May 2009 diff hist +92 N Bridges Lab Protocols:About Created page with 'This page contains protocols developed by and for use in the laboratory of Dr. Alan Saltiel.'
• 00:5800:58, 2 May 2009 diff hist +58 Main Page No edit summary
• 00:5800:58, 2 May 2009 diff hist +1,476 N Restriction Enzyme Based Cloning - Ordering Primers Created page with '==Preparation and Ordering Primers== #Choose target vector and region of interest in gene #Pick 5' and 3' restriction sites that are present in the vector but NOT in the insert #...'
• 00:5400:54, 2 May 2009 diff hist +32 Western Blotting →Protocol
• 00:5300:53, 2 May 2009 diff hist +4 Western Blotting →Materials
• 00:5100:51, 2 May 2009 diff hist +22 Main Page No edit summary
• 00:5100:51, 2 May 2009 diff hist +1,232 N Western Blotting Created page with '==Materials== *Transfer Buffer (100 mL Methanol, 50 mL 10X Transfer Buffer to final 500 mL) *Transfer Apparatus, either Bio-Rad or Invitrogen ==Protocol== #Run SDS-PAGE gel and ...'
• 00:4500:45, 2 May 2009 diff hist +18 Main Page →Cloning and Molecular Biology
• 00:4500:45, 2 May 2009 diff hist +611 N TOPO Cloning Created page with '#PCR desired insert using Pfu polymerase. Add 1 uL of Taq and mix. Incubate 10min at 72C to add A to 3’ ends. Use immediately #If PCR product is a single band, no need to pu...' current
• 00:4000:40, 2 May 2009 diff hist +60 Main Page No edit summary
• 00:3900:39, 2 May 2009 diff hist +41 Surface Plasmon Resonance - Protein Lipid Interactions No edit summary
• 00:3500:35, 2 May 2009 diff hist +1,640 N Surface Plasmon Resonance - Protein Lipid Interactions Created page with '==Materials== *L1 Sensor Chip (Biacore) *Dioleylphosphatidylcholine (Sigma) dissolved in 1:1 chloroform:methanol with 0.1% HCl *PIPx of interest (Avanti or Echelon) dissolved in ...'
• 00:3100:31, 2 May 2009 diff hist +55 Main Page →Cloning and Molecular Biology
• 00:3100:31, 2 May 2009 diff hist +1,020 N Restriction Enzyme Based Cloning copied over protocol
• 00:2900:29, 2 May 2009 diff hist +13 Mutagenesis →Protocol
• 00:2900:29, 2 May 2009 diff hist 0 Mutagenesis →Protocol
• 00:2800:28, 2 May 2009 diff hist +1,049 N Mutagenesis copied over protocol
• 00:0800:08, 2 May 2009 diff hist +23 Main Page →Cell and Tissue Culture
• 00:0800:08, 2 May 2009 diff hist +1 Immunofluoresence No edit summary
• 00:0700:07, 2 May 2009 diff hist +1,242 N Immunofluoresence copied over protocol

1 May 2009

• 23:5823:58, 1 May 2009 diff hist +2 m Main Page →Transcriptional Analysis
• 23:5723:57, 1 May 2009 diff hist −273 Main Page →Getting started: removed section
• 22:5322:53, 1 May 2009 diff hist +621 N Bradford Assay copied over protocol
• 22:5022:50, 1 May 2009 diff hist +109 Main Page No edit summary
• 22:4822:48, 1 May 2009 diff hist +567 N Transformation of Bacteria copied over protocol
• 22:2622:26, 1 May 2009 diff hist +2,942 N Differentiation of 3T3-L1 Cells copied over page
• 22:2322:23, 1 May 2009 diff hist +81 Main Page →Protocols: added links
• 20:0220:02, 1 May 2009 diff hist +26 Real Time PCR From Cell Culture →References (Saltiel Lab): added Chang et al Mol Endocrinol
• 20:0020:00, 1 May 2009 diff hist +31 N Help:Contents wrote initial help page
• 19:5119:51, 1 May 2009 diff hist +78 Real Time PCR From Cell Culture →References (Saltiel Lab): added references
• 19:4519:45, 1 May 2009 diff hist −62 m Main Page No edit summary
• 19:4419:44, 1 May 2009 diff hist +53 Main Page No edit summary
• 19:4119:41, 1 May 2009 diff hist +31 Real Time PCR From Cell Culture No edit summary
• 19:3919:39, 1 May 2009 diff hist +7,157 N Help:Editing Created page with 'from http://meta.wikimedia.org/wiki/Help:Editing#Wiki_markups_and_codes {{H:h|editor toc}} '''Editing''' refers to the changing of a page by a Wikimedia user. Unless certain re...' current
• 19:3719:37, 1 May 2009 diff hist +1,129 N Real Time PCR From Cell Culture Created page with 'Real Time qPCR Materials cDNA for templates Qiashredder and RNEasy kits from Qiagen Superscript Kit from Invitrogen SyberGreen PCR Master Mix Applied Biosystems 96 well qPCR plat...'