TaqMan SNP Assay: Difference between revisions
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*TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems). | *TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems). | ||
* | *TaqMan® Genotyping Master Mix (supplied at 2X)(Applied Biosystems). | ||
*StepOnePlus Real-Time PCR System (Applied Biosystems). | *StepOnePlus Real-Time PCR System (Applied Biosystems). | ||
*PC/Laptop with StepOnePlus Software installed. | *PC/Laptop with StepOnePlus Software installed. | ||
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== Protocol == | == Protocol == | ||
# Dilute gDNA samples to 1-5 ng/ul. | |||
# Place gDNA, TaqMan® Genotyping Master Mix, TaqMan® Genotyping Assay on ice. | |||
# Make up Reaction Master mix (see table below). | |||
Revision as of 17:20, 12 November 2015
Materials
- TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems).
- TaqMan® Genotyping Master Mix (supplied at 2X)(Applied Biosystems).
- StepOnePlus Real-Time PCR System (Applied Biosystems).
- PC/Laptop with StepOnePlus Software installed.
- MicroAmp Fast 96-Well Plates.
- Optical Plate Covers.
- Nuclease-Free Water
- Sample Genomic DNA (extracted as described in Blood DNA Extraction)
- Postive control(s) (Minor allele) (optional, but recommended)
Protocol
- Dilute gDNA samples to 1-5 ng/ul.
- Place gDNA, TaqMan® Genotyping Master Mix, TaqMan® Genotyping Assay on ice.
- Make up Reaction Master mix (see table below).