TaqMan SNP Assay: Difference between revisions
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*Optical Plate Covers. | *Optical Plate Covers. | ||
*Nuclease-Free Water | *Nuclease-Free Water | ||
*Sample Genomic DNA | *Sample Genomic DNA (extracted as described ) | ||
*Postive control(s) (Minor allele) (''optional, but recommended'') | *Postive control(s) (Minor allele) (''optional, but recommended'') | ||
== Protocol == | == Protocol == | ||
Revision as of 16:15, 11 November 2015
Materials
- TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems).
- TaqMan Genotyping Master Mix (supplied at 2X)(Applied Biosystems).
- StepOnePlus Real-Time PCR System (Applied Biosystems).
- PC/Laptop with StepOnePlus Software installed.
- MicroAmp Fast 96-Well Plates.
- Optical Plate Covers.
- Nuclease-Free Water
- Sample Genomic DNA (extracted as described )
- Postive control(s) (Minor allele) (optional, but recommended)