TaqMan SNP Assay: Difference between revisions

From Bridges Lab Protocols
Jump to navigation Jump to search
Lmcallan (talk | contribs)
No edit summary
Lmcallan (talk | contribs)
No edit summary
Line 2: Line 2:
== Materials ==
== Materials ==


TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X).
TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems).


TaqMan Genotyping Master Mix (supplied at 2X).
TaqMan Genotyping Master Mix (supplied at 2X)(Applied Biosystems).


MicroAmp Fast 96-Well Plates.
MicroAmp Fast 96-Well Plates.


Optical Plate Covers.
Optical Plate Covers.
StepOnePlus Real-Time PCR System (Applied Biosystems).
PC/Laptop with StepOnePlus Software installed




== Protocol ==
== Protocol ==

Revision as of 15:55, 11 November 2015

Materials

TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems).

TaqMan Genotyping Master Mix (supplied at 2X)(Applied Biosystems).

MicroAmp Fast 96-Well Plates.

Optical Plate Covers.

StepOnePlus Real-Time PCR System (Applied Biosystems).

PC/Laptop with StepOnePlus Software installed


Protocol