Cre PCR: Difference between revisions

From Bridges Lab Protocols
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*72C 5min
*72C 5min
*4C forever
*4C forever
[[ Category:Genotyping ]]
[[ Category:Mouse Work]]
[[ Category:PCR]]
[[ Category:DNA]]
[[ Category:Molecular Biology]]

Revision as of 14:37, 9 July 2013

PCR Program

  • 95C 1min
  • 35 Cycles of:
    • 95C 10s
    • 55C 30s
    • 72C 45s
  • 72C 5min
  • 4C forever