Immunoprecipitation: Difference between revisions
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Created page with 'Lyse cells in lysis buffer, such as RIPA buffer with protease inhibitors and phosphatase inhibitors (see link). For each well in a 12 well plate lyse in 1 ml. rotate 0.5 ml lysat...' |
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Lyse cells in lysis buffer, such as RIPA buffer with protease inhibitors and phosphatase inhibitors (see | ==Protocol== | ||
For each well in a 12 well plate lyse in 1 ml. | * Lyse cells in lysis buffer, such as RIPA buffer with protease inhibitors and phosphatase inhibitors (see [[RIPA Buffer]]). | ||
rotate 0.5 ml lysate with 25 ul of protein A/G plus agarose beads (catalog # sc 2003 santa cruz). | * For each well in a 12 well plate lyse in 1 ml. | ||
* rotate 0.5 ml lysate with 25 ul of protein A/G plus agarose beads (catalog # sc 2003 santa cruz). | |||
Revision as of 17:12, 4 April 2012
Protocol
- Lyse cells in lysis buffer, such as RIPA buffer with protease inhibitors and phosphatase inhibitors (see RIPA Buffer).
- For each well in a 12 well plate lyse in 1 ml.
- rotate 0.5 ml lysate with 25 ul of protein A/G plus agarose beads (catalog # sc 2003 santa cruz).