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==Protein Analysis== | ==Protein Analysis== | ||
*[[Preparing a SDS-PAGE Gel]] | |||
*[[Western Blotting]] | *[[Western Blotting]] | ||
*[[Surface Plasmon Resonance - Protein Lipid Interactions]] | *[[Surface Plasmon Resonance - Protein Lipid Interactions]] | ||
Revision as of 18:58, 3 August 2009
Cell and Tissue Culture
- Splitting Cells
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Fugene Transfection of 293T/COS Cells
- Lipofectamine Mediated Knockdown
- Immunofluoresence
- 3T3-L1 Adipocyte Fractionation
- Preparing Cell Lysates
- Glucose Uptake Assay
- Luciferase Assay
Cloning and Molecular Biology
- Transformation of Bacteria
- Mutagenesis
- PCR Amplification of DNA
- Restriction Enzyme Based Cloning
- TOPO Cloning
- Preparing an Agarose Gel
- Cesium Chloride Preparation of DNA
Protein Analysis
Protein Quantification
Protein Purification
- French Press
- Purification of GST Fusion Proteins
- Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
Transcriptional Analysis
Mouse Protocols
Genotyping
Metabolic Measurements
Tissue Preparation
- Harvesting Mouse Tissue
- Preparation of Protein Lysates from Mouse Tissues
- Preparation of RNA Samples from Mouse Tissues