Designing and Preparing dsRNA: Difference between revisions
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*Add T7 RNA Polymerase sites to the 5' of each primer ('''TAATACGACTCACTATAGG''') | *Add T7 RNA Polymerase sites to the 5' of each primer ('''TAATACGACTCACTATAGG''') | ||
*Record primer and amplicon (DSRC) | *Record primer and amplicon (DSRC) | ||
==Preparing dsRNA== | |||
*see http://www.flyrnai.org/DRSC-PRS.html | |||
Revision as of 13:47, 3 August 2009
Ordering dsRNA Primers
- If available, choose primers from Harvard DSRC site http://www.flyrnai.org/cgi-bin/RNAi_gene_lookup_public.pl
- Add T7 RNA Polymerase sites to the 5' of each primer (TAATACGACTCACTATAGG)
- Record primer and amplicon (DSRC)