Measuring Fasting Insulin: Difference between revisions

From Bridges Lab Protocols
Jump to navigation Jump to search
← Older editNewer edit →
Updated with product details
No edit summary
Line 1: Line 1:
==Materials==
==Materials==
*Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)
*Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)
*Heparinzed capilaries (Fisher cat# 22-362574)


==Protocol==
==Protocol==
#Collect about 2/3 of a capillary of blood, typically via a retro-orbital bleed.
#Collect serum (see [[Collecting_and_Storing_Mouse_Serum]])
#Transfer blood to labelled eppendorf tubes.
#Let blood clot for 30 min on ice.
#Centrifuge 20 min at 2000g (about 5000 RPM in eppendorf 5415C centrifuge).
#Gentry remove liquid layer and store at -80

Revision as of 15:54, 18 January 2024

Materials

  • Insulin ELISA (Ultra Sensitive Mouse Insulin ELISA Kit from Crystal Chem catalog # 90080)

Protocol

  1. Collect serum (see Collecting_and_Storing_Mouse_Serum)
Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php?title=Measuring_Fasting_Insulin&oldid=2687"