From Bridges Lab Protocols
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| == Cholesterol Assay ==
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| === Materials ===
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| * Reagent
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| * Cholesterol Standard
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| === Protocol ===
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| * Make 10X stock dilution: Add 50ɥL Cholesterol Standard 450ɥL water to a 1.5mL tube
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| * Pipette 100ɥL of Reagent to 22 wells
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| * Add 2-10ɥL of 10X stock dilution to wells in columns 2-6 in increments of 2ɥL. Each column will have 2 replicates per volume (1 replicate per row).
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| * Repeat in columns 7-11 with 2-10ɥL of Cholesterol Standard (1X dilution)
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| * Allow reaction to proceed for 15 minutes
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| * Read absorbance
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Latest revision as of 13:50, 28 October 2023