FDB Muscle Dissection from Mice: Difference between revisions
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This protocol is modified from section 3.2 | This protocol is modified from section 3.1.2 of [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3523695/ Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells] and the first part of the following video with accompanying protocol [https://www.jove.com/video/53551/dna-electroporation-isolation-and-imaging-of-myofibers DNA Electroporation, Isolation and Imaging of Myofibers | Protocol]. | ||
<big>'''Materials'''</big> | <big>'''Materials'''</big> | ||
* | * Tweezers | ||
* Standard operating scissors | |||
* Surgical scissors | |||
* Forceps | |||
* Dissection stage with 7 pins | |||
* Anesthetic | |||
<big>'''Flexor Digitorum Brevis Muscle Extraction'''</big> | |||
* Sacrifice the animal with anesthetic gas inhalation followed by cervical dislocation to ensure death. | |||
* | |||
Revision as of 19:08, 13 June 2017
This protocol is modified from section 3.1.2 of Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells and the first part of the following video with accompanying protocol DNA Electroporation, Isolation and Imaging of Myofibers | Protocol.
Materials
- Tweezers
- Standard operating scissors
- Surgical scissors
- Forceps
- Dissection stage with 7 pins
- Anesthetic
Flexor Digitorum Brevis Muscle Extraction
- Sacrifice the animal with anesthetic gas inhalation followed by cervical dislocation to ensure death.