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*[[Bradford Assay]] | *[[Bradford Assay]] | ||
*[[Quantification by Absorbance at 280nm]] | *[[Quantification by Absorbance at 280nm]] | ||
*[[Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)]] | |||
===Protein Purification=== | ===Protein Purification=== | ||
Revision as of 20:27, 19 May 2009
Cell and Tissue Culture
- Splitting Cells
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Immunofluoresence
- 3T3-L1 Adipocyte Fractionation
- Preparing Cell Lysates
- Harvesting RNA from Cells grown in monolayer
Cloning and Molecular Biology
- Transformation of Bacteria
- Mutagenesis
- PCR Amplification of DNA
- Restriction Enzyme Based Cloning
- TOPO Cloning
- Preparing an Agarose Gel
Protein Analysis
Protein Quantification
- Bradford Assay
- Quantification by Absorbance at 280nm
- Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)