Preparing an Agarose Gel: Difference between revisions
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| Line 5: | Line 5: | ||
==Prepare Gel (BioRad Gel Rig)== | ==Prepare Gel (BioRad Gel Rig)== | ||
#Place plastic mold in holder | #Place plastic mold securely in holder | ||
#Put | #Put well comb in mold | ||
#Weight out | #Weight out 1.0g agarose and add 100 mL of TAE (1% solution) then return TAE to shelf | ||
#Microwave | #Microwave 90s until dissolved | ||
#Add | #Add 2 uL Gel Red. Swirl to mix and eliminate sediments | ||
#Pour into mold | #Pour into mold | ||
#Place gel in cold room if in a hurry | #Place gel in cold room if in a hurry | ||
#After loading, run gel at 100V | #After loading, run gel at 100V | ||
Latest revision as of 19:22, 24 August 2016
Materials
- Agarose
- TAE Buffer (1X): Dilute from 100X stock
- Thermo Apparatus (Mold, Holder, Comb and Gel Rig)
Prepare Gel (BioRad Gel Rig)
- Place plastic mold securely in holder
- Put well comb in mold
- Weight out 1.0g agarose and add 100 mL of TAE (1% solution) then return TAE to shelf
- Microwave 90s until dissolved
- Add 2 uL Gel Red. Swirl to mix and eliminate sediments
- Pour into mold
- Place gel in cold room if in a hurry
- After loading, run gel at 100V