PCR Analysis of Tail DNA: Difference between revisions
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==Materials== | ==Materials== | ||
# Dream Taq Green master mix | # Dream Taq Green master mix | ||
# Specific Primers | # Specific gene Primers (0.4um Working stock) | ||
# DNA | # Tail digest DNA | ||
# ddH2O | # ddH2O | ||
Revision as of 18:39, 17 February 2016
see Genotyping Details for strain specific details
Materials
- Dream Taq Green master mix
- Specific gene Primers (0.4um Working stock)
- Tail digest DNA
- ddH2O
Protocol
Use the following Volumes per 25ul Reaction:
Per sample 1X
- Dream Tag Master mix: 12.5uL ("Molecular Biology Stuff" box in freezer)
- Primer Mix: 5ul
- Sterile ddH2O: 7.5ul
- Template: 1 uL
Run "specfic" PCR Program for gene of interest (approx 2 hours).
see Preparing an Agarose Gel for details on preparing a DNA gel