Induction Conditions: Difference between revisions
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added hrs and fyve |
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|- | |- | ||
! Protein | ! Protein | ||
! IPTG | ! IPTG | ||
! Time | ! Time | ||
! Temperature | ! Temperature | ||
! Notes | ! Notes | ||
|- | |- | ||
| | | GST-PLC | ||
| | | 10 uM | ||
| | | Overnight | ||
| | | 28C | ||
| | | very soluble and easy to induce. | ||
|- | |||
| GST-HRS | |||
| 10 uM | |||
| Overnight | |||
| 28C | |||
| very soluble and easy to induce. | |||
|- | |||
| GST-HRS (2xFYVE) | |||
| 10 uM | |||
| Overnight | |||
| 28C | |||
| very soluble and easy to induce. | |||
|} | |} | ||
Revision as of 14:36, 12 August 2009
| Protein | IPTG | Time | Temperature | Notes |
|---|---|---|---|---|
| GST-PLC | 10 uM | Overnight | 28C | very soluble and easy to induce. |
| GST-HRS | 10 uM | Overnight | 28C | very soluble and easy to induce. |
| GST-HRS (2xFYVE) | 10 uM | Overnight | 28C | very soluble and easy to induce. |