Genotyping Program: Difference between revisions
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Updated table, added links to specific genotyping protocols |
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!| Allele || Program|| Fragment Size(s) || Notes | !| Allele || Program|| Fragment Size(s) || Notes | ||
|- | |- | ||
|TSC-floxed || Inoki PCR || || Two bands if heterozygote | |[[TSC-floxed Genotyping| TSC-floxed]]|| Inoki PCR || || Two bands if heterozygote | ||
|- | |- | ||
|Cre || Inoki PCR || || Only present if Transgene Positive (cannot detect homozygosity at transgene) | |[[Cre Genotyping|Cre]] || Inoki PCR || || Only present if Transgene Positive (cannot detect homozygosity at transgene) | ||
|- | |- | ||
|Vac14 || Inoki PCR|| || Two bands if heterozygote | |[[Vac14 Genotyping|Vac14]] || Inoki PCR|| || Two bands if heterozygote | ||
|- | |- | ||
|TC10b || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote | |[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote | ||
|- | |||
|[[CAP Genotyping|CAP]] || CAP || || Two bands if heterozygote | |||
|} | |} | ||
[[ Category:Genotyping ]] | [[ Category:Genotyping ]] | ||
[[ Category:Mouse Work]] | [[ Category:Mouse Work]] | ||
[[ Category:PCR]] | [[ Category:PCR]] | ||
Revision as of 17:23, 27 February 2012
| Allele | Program | Fragment Size(s) | Notes |
|---|---|---|---|
| TSC-floxed | Inoki PCR | Two bands if heterozygote | |
| Cre | Inoki PCR | Only present if Transgene Positive (cannot detect homozygosity at transgene) | |
| Vac14 | Inoki PCR | Two bands if heterozygote | |
| TC10b | TC10b | KO - 500bp, WT-1kb | Two bands if heterozygote |
| CAP | CAP | Two bands if heterozygote |