Designing and Preparing dsRNA: Difference between revisions
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==Ordering dsRNA Primers== | ==Ordering dsRNA Primers== | ||
*If available choose primers from Harvard DSRC site http://www.flyrnai.org/ | *If available, choose primers from Harvard DSRC site http://www.flyrnai.org/cgi-bin/RNAi_gene_lookup_public.pl | ||
*Add T7 RNA Polymerase sites to the 5' of each primer | *Add T7 RNA Polymerase sites to the 5' of each primer ('''TAATACGACTCACTATAGG''') | ||
'''TAATACGACTCACTATAGG''' | *Record primer and amplicon (DSRC) | ||
Revision as of 13:44, 3 August 2009
Ordering dsRNA Primers
- If available, choose primers from Harvard DSRC site http://www.flyrnai.org/cgi-bin/RNAi_gene_lookup_public.pl
- Add T7 RNA Polymerase sites to the 5' of each primer (TAATACGACTCACTATAGG)
- Record primer and amplicon (DSRC)